Document Detail

Thermophilic sulfate reduction and methanogenesis with methanol in a high rate anaerobic reactor.
MedLine Citation:
PMID:  10620266     Owner:  NLM     Status:  MEDLINE    
Sulfate reduction outcompeted methanogenesis at 65 degrees C and pH 7.5 in methanol and sulfate-fed expanded granular sludge bed reactors operated at hydraulic retention times (HRT) of 14 and 3.5 h, both under methanol-limiting and methanol-overloading conditions. After 100 and 50 days for the reactors operated at 14 and 3.5 h, respectively, sulfide production accounted for 80% of the methanol-COD consumed by the sludge. The specific methanogenic activity on methanol of the sludge from a reactor operated at HRTs of down to 3.5 h for a period of 4 months gradually decreased from 0. 83 gCOD. gVSS(-1). day(-1) at the start to a value of less than 0.05 gCOD. gVSS(-1). day(-1), showing that the relative number of methanogens decreased and eventually became very low. By contrast, the increase of the specific sulfidogenic activity of sludge from 0. 22 gCOD. gVSS(-1). day(-1) to a final value of 1.05 gCOD. gVSS(-1). day(-1) showed that sulfate reducing bacteria were enriched. Methanol degradation by a methanogenic culture obtained from a reactor by serial dilution of the sludge was inhibited in the presence of vancomycin, indicating that methanogenesis directly from methanol was not important. H(2)/CO(2) and formate, but not acetate, were degraded to methane in the presence of vancomycin. These results indicated that methanol degradation to methane occurs via the intermediates H(2)/CO(2) and formate. The high and low specific methanogenic activity of sludge on H(2)/CO(2) and formate, respectively, indicated that the former substrate probably acts as the main electron donor for the methanogens during methanol degradation. As sulfate reduction in the sludge was also strongly supported by hydrogen, competition between sulfate reducing bacteria and methanogens in the sludge seemed to be mainly for this substrate. Sulfate elimination rates of up to 15 gSO(4)(2-)/L per day were achieved in the reactors. Biomass retention limited the sulfate elimination rate.
J Weijma; A J Stams; L W Hulshoff Pol; G Lettinga
Related Documents :
23061816 - Two new pregnane glycoside diesters from caralluma russeliana.
24197896 - Heavy metal content of moss from kampinos national park in poland.
17258796 - Estimating national exhaust emissions from railway vehicles in turkey.
20154026 - Recycling waste brick from construction and demolition of buildings as pozzolanic mater...
22349346 - Quantification of ligand packing density on gold nanoparticles using icp-oes.
21386376 - Superconductivity at 23 k in pt doped bafe(2)as(2) single crystals.
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biotechnology and bioengineering     Volume:  67     ISSN:  0006-3592     ISO Abbreviation:  Biotechnol. Bioeng.     Publication Date:  2000 Feb 
Date Detail:
Created Date:  2000-02-15     Completed Date:  2000-02-15     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  7502021     Medline TA:  Biotechnol Bioeng     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  354-63     Citation Subset:  IM    
Copyright Information:
Copyright 2000 John Wiley & Sons, Inc.
Department of Biomolecular Sciences, Laboratory of Microbiology, Wageningen University, Hesselink van Suchtelenweg 4, 6703 CT, Wageningen, The Netherlands.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Biotechnology / instrumentation,  methods
Carbon / metabolism
Carbon Dioxide
Energy Metabolism
Hot Temperature*
Methane / metabolism*
Methanol / metabolism*
Sewage / chemistry,  microbiology
Sulfates / metabolism*
Reg. No./Substance:
0/Acetates; 0/Formates; 0/Sewage; 0/Sulfates; 124-38-9/Carbon Dioxide; 1333-74-0/Hydrogen; 67-56-1/Methanol; 74-82-8/Methane; 7440-44-0/Carbon

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Hepatocyte behavior within three-dimensional porous alginate scaffolds.
Next Document:  The nature of fatty acid modifies the equilibrium position in the esterification catalyzed by lipase...