| Thermophilic sulfate reduction and methanogenesis with methanol in a high rate anaerobic reactor. | |
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MedLine Citation:
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PMID: 10620266 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Sulfate reduction outcompeted methanogenesis at 65 degrees C and pH 7.5 in methanol and sulfate-fed expanded granular sludge bed reactors operated at hydraulic retention times (HRT) of 14 and 3.5 h, both under methanol-limiting and methanol-overloading conditions. After 100 and 50 days for the reactors operated at 14 and 3.5 h, respectively, sulfide production accounted for 80% of the methanol-COD consumed by the sludge. The specific methanogenic activity on methanol of the sludge from a reactor operated at HRTs of down to 3.5 h for a period of 4 months gradually decreased from 0. 83 gCOD. gVSS(-1). day(-1) at the start to a value of less than 0.05 gCOD. gVSS(-1). day(-1), showing that the relative number of methanogens decreased and eventually became very low. By contrast, the increase of the specific sulfidogenic activity of sludge from 0. 22 gCOD. gVSS(-1). day(-1) to a final value of 1.05 gCOD. gVSS(-1). day(-1) showed that sulfate reducing bacteria were enriched. Methanol degradation by a methanogenic culture obtained from a reactor by serial dilution of the sludge was inhibited in the presence of vancomycin, indicating that methanogenesis directly from methanol was not important. H(2)/CO(2) and formate, but not acetate, were degraded to methane in the presence of vancomycin. These results indicated that methanol degradation to methane occurs via the intermediates H(2)/CO(2) and formate. The high and low specific methanogenic activity of sludge on H(2)/CO(2) and formate, respectively, indicated that the former substrate probably acts as the main electron donor for the methanogens during methanol degradation. As sulfate reduction in the sludge was also strongly supported by hydrogen, competition between sulfate reducing bacteria and methanogens in the sludge seemed to be mainly for this substrate. Sulfate elimination rates of up to 15 gSO(4)(2-)/L per day were achieved in the reactors. Biomass retention limited the sulfate elimination rate. |
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Authors:
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J Weijma; A J Stams; L W Hulshoff Pol; G Lettinga |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Biotechnology and bioengineering Volume: 67 ISSN: 0006-3592 ISO Abbreviation: Biotechnol. Bioeng. Publication Date: 2000 Feb |
Date Detail:
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Created Date: 2000-02-15 Completed Date: 2000-02-15 Revised Date: 2008-11-21 |
Medline Journal Info:
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Nlm Unique ID: 7502021 Medline TA: Biotechnol Bioeng Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 354-63 Citation Subset: IM |
Copyright Information:
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Copyright 2000 John Wiley & Sons, Inc. |
Affiliation:
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Department of Biomolecular Sciences, Laboratory of Microbiology, Wageningen University, Hesselink van Suchtelenweg 4, 6703 CT, Wageningen, The Netherlands. jan.weijma@algemeen.mt.wau.nl |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Acetates Anaerobiosis Bioreactors* Biotechnology / instrumentation, methods Carbon / metabolism Carbon Dioxide Energy Metabolism Formates Hot Temperature* Hydrogen Methane / metabolism* Methanol / metabolism* Pressure Sewage / chemistry, microbiology Sulfates / metabolism* |
| Chemical | |
Reg. No./Substance:
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0/Acetates; 0/Formates; 0/Sewage; 0/Sulfates; 124-38-9/Carbon Dioxide; 1333-74-0/Hydrogen; 67-56-1/Methanol; 74-82-8/Methane; 7440-44-0/Carbon |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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