Document Detail


Thermophilic sulfate reduction and methanogenesis with methanol in a high rate anaerobic reactor.
MedLine Citation:
PMID:  10620266     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Sulfate reduction outcompeted methanogenesis at 65 degrees C and pH 7.5 in methanol and sulfate-fed expanded granular sludge bed reactors operated at hydraulic retention times (HRT) of 14 and 3.5 h, both under methanol-limiting and methanol-overloading conditions. After 100 and 50 days for the reactors operated at 14 and 3.5 h, respectively, sulfide production accounted for 80% of the methanol-COD consumed by the sludge. The specific methanogenic activity on methanol of the sludge from a reactor operated at HRTs of down to 3.5 h for a period of 4 months gradually decreased from 0. 83 gCOD. gVSS(-1). day(-1) at the start to a value of less than 0.05 gCOD. gVSS(-1). day(-1), showing that the relative number of methanogens decreased and eventually became very low. By contrast, the increase of the specific sulfidogenic activity of sludge from 0. 22 gCOD. gVSS(-1). day(-1) to a final value of 1.05 gCOD. gVSS(-1). day(-1) showed that sulfate reducing bacteria were enriched. Methanol degradation by a methanogenic culture obtained from a reactor by serial dilution of the sludge was inhibited in the presence of vancomycin, indicating that methanogenesis directly from methanol was not important. H(2)/CO(2) and formate, but not acetate, were degraded to methane in the presence of vancomycin. These results indicated that methanol degradation to methane occurs via the intermediates H(2)/CO(2) and formate. The high and low specific methanogenic activity of sludge on H(2)/CO(2) and formate, respectively, indicated that the former substrate probably acts as the main electron donor for the methanogens during methanol degradation. As sulfate reduction in the sludge was also strongly supported by hydrogen, competition between sulfate reducing bacteria and methanogens in the sludge seemed to be mainly for this substrate. Sulfate elimination rates of up to 15 gSO(4)(2-)/L per day were achieved in the reactors. Biomass retention limited the sulfate elimination rate.
Authors:
J Weijma; A J Stams; L W Hulshoff Pol; G Lettinga
Related Documents :
20550206 - Recoupled pair bonding in pf(n) (n = 1-5).
19002326 - Interactions of 1,5-naphthyridine with pd(en)cl2 or [pd(en)(h2o)2](no3)2 in aqueous sol...
18940316 - Rapid quantitative determination of hydrogen peroxide by oxidation decolorization of me...
9528686 - Reactions of halogen-substituted aziridinylbenzoquinones with glutathione. formation of...
21795796 - Crystallization and preliminary crystallographic analysis of the phosphotriesterase-lik...
20858006 - Bimetallic reductive elimination from dinuclear pd(iii) complexes.
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biotechnology and bioengineering     Volume:  67     ISSN:  0006-3592     ISO Abbreviation:  Biotechnol. Bioeng.     Publication Date:  2000 Feb 
Date Detail:
Created Date:  2000-02-15     Completed Date:  2000-02-15     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  7502021     Medline TA:  Biotechnol Bioeng     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  354-63     Citation Subset:  IM    
Copyright Information:
Copyright 2000 John Wiley & Sons, Inc.
Affiliation:
Department of Biomolecular Sciences, Laboratory of Microbiology, Wageningen University, Hesselink van Suchtelenweg 4, 6703 CT, Wageningen, The Netherlands. jan.weijma@algemeen.mt.wau.nl
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Acetates
Anaerobiosis
Bioreactors*
Biotechnology / instrumentation,  methods
Carbon / metabolism
Carbon Dioxide
Energy Metabolism
Formates
Hot Temperature*
Hydrogen
Methane / metabolism*
Methanol / metabolism*
Pressure
Sewage / chemistry,  microbiology
Sulfates / metabolism*
Chemical
Reg. No./Substance:
0/Acetates; 0/Formates; 0/Sewage; 0/Sulfates; 124-38-9/Carbon Dioxide; 1333-74-0/Hydrogen; 67-56-1/Methanol; 74-82-8/Methane; 7440-44-0/Carbon

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Hepatocyte behavior within three-dimensional porous alginate scaffolds.
Next Document:  The nature of fatty acid modifies the equilibrium position in the esterification catalyzed by lipase...