Document Detail


Thermogenesis induced by osmotic stimulation of the intestines in the rat.
MedLine Citation:
PMID:  11283240     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Infusion of 5-20% glucose, 1.8-3.6% NaCl, 20% methylglucose, 20% fructose, or 5-10% solutions of various amino acids (10 ml x kg(-1)) into the duodenum induced dose-dependent thermogenesis in urethane-anaesthetized rats. In contrast, infusion of 0.9% NaCl, distilled water, or safflower oil had no effect on the metabolic rate. Infusion of 7.2% urea induced a small and transient increase in the metabolic rate. These results suggested that the thermogenesis was caused mainly by changes in osmolality rather than by a specific action of the different solute molecules. The respiratory exchange ratio increased after the infusion of glucose, fructose, glycine, or serine, did not change after the infusion of NaCl, methylglucose, safflower oil, or distilled water, and decreased after infusion of arginine. Therefore, there was no relationship between substrate utilization and the occurrence of thermogenesis. Intestinal infusion of 3.6% NaCl elevated the plasma osmolality, with a plateau increase of approximately 20 mosmol x kg(-1). However, intravenous infusion of the same amount of NaCl induced a significantly smaller thermogenic response, although it elevated the plasma osmolality with a time course and magnitude similar to those obtained after the intestinal infusion. Infusion of NaCl into the hepatic portal vein or the peritoneal cavity also produced a significantly small thermogenic response. These results suggested an intestinal or mesenteric location for osmoreceptors. To test for possible stimulation of intestinal osmoreceptors after intake of a normal meal, we measured the osmolality of the intestinal contents. The osmolality of the duodeno-jejunal contents was 600-800 mosmol kg-1, whereas the plasma osmolality was 306 +/- 1 mosmol x kg(-1), which suggests that the intestinal osmoreceptors are stimulated after meals and are involved in diet-induced thermogenesis.
Authors:
T Osaka; A Kobayashi; S Inoue
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Journal of physiology     Volume:  532     ISSN:  0022-3751     ISO Abbreviation:  J. Physiol. (Lond.)     Publication Date:  2001 Apr 
Date Detail:
Created Date:  2001-04-03     Completed Date:  2001-08-02     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  0266262     Medline TA:  J Physiol     Country:  England    
Other Details:
Languages:  eng     Pagination:  261-9     Citation Subset:  IM    
Affiliation:
National Institute of Health and Nutrition, Shinjuku 162-8636, Japan. osaka@nih.gov.jp
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MeSH Terms
Descriptor/Qualifier:
Amino Acids / administration & dosage,  metabolism
Animals
Basal Metabolism / physiology*
Body Temperature*
Dose-Response Relationship, Drug
Fructose / administration & dosage,  metabolism
Gastrointestinal Contents / chemistry*
Glucose / administration & dosage,  chemistry,  metabolism
Humans
Infusions, Intravenous
Intestines / physiology*,  surgery
Male
Osmolar Concentration*
Rats
Rats, Wistar
Sodium Chloride / administration & dosage,  metabolism
Thermogenesis / physiology*
Urea / administration & dosage,  metabolism
Chemical
Reg. No./Substance:
0/Amino Acids; 30237-26-4/Fructose; 50-99-7/Glucose; 57-13-6/Urea; 7647-14-5/Sodium Chloride
Comments/Corrections

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