Document Detail


Thermodynamic and structural behaviour of equimolar POPC/CnE4 (n=8, 12, 16) mixtures by sorption gravimetry, 2H NMR spectroscopy and X-ray diffraction.
MedLine Citation:
PMID:  20060820     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The hydration behaviour of equimolar mixtures of phospholipids and nonionic surfactants with different chain length was investigated by gravimetric sorption, NMR spectroscopy and X-ray diffraction. At the most hydration degrees investigated, the incorporation of nonionic surfactants in a phospholipid bilayer leads to an increase of the hydrophilicity, which can be shown by the presence of excess hydration. The increased hydrophilicity could be explained by the excavation of additional water binding sites due to the "dilution" of the dipole field of the phospholipid bilayer. Another related contribution arises from the increase of the accessible surface area due to the increase of gauche conformers that result from the steric mismatch when surfactants are incorporated into the phospholipid matrix. (2)H NMR spectroscopy was used to determine the quadrupolar splitting representing a measure of the order state of water. The swelling behaviour could be assessed by small-angle X-ray diffraction. (31)P NMR spectroscopy was applied for the assignment of phase structures to the respective hydration range.
Authors:
H Pfeiffer; G Klose; K Heremans
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-01-09
Journal Detail:
Title:  Chemistry and physics of lipids     Volume:  163     ISSN:  1873-2941     ISO Abbreviation:  Chem. Phys. Lipids     Publication Date:  2010 Mar 
Date Detail:
Created Date:  2010-03-01     Completed Date:  2010-06-01     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0067206     Medline TA:  Chem Phys Lipids     Country:  Ireland    
Other Details:
Languages:  eng     Pagination:  318-28     Citation Subset:  IM    
Copyright Information:
Copyright 2010 Elsevier Ireland Ltd. All rights reserved.
Affiliation:
Katholieke Universiteit Leuven, Department of Metallurgy and Materials Engineering, B-3001 Leuven, Belgium. Helge.Pfeiffer@mtm.kuleuven.be
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MeSH Terms
Descriptor/Qualifier:
Lipid Bilayers
Magnetic Resonance Spectroscopy / methods*
Molecular Structure
Phosphatidylcholines / chemistry*
Thermodynamics*
X-Ray Diffraction / methods*
Chemical
Reg. No./Substance:
0/Lipid Bilayers; 0/Phosphatidylcholines; 6753-55-5/1-palmitoyl-2-oleoylphosphatidylcholine

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