Document Detail


Thermally induced myocardial preservation and necrosis in deprived fetal mouse hearts.
MedLine Citation:
PMID:  842685     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Cultured fetal mouse hearts deprived of oxygen and glucose were used to examine the effect of temperature on the mechanical, biochemical, and ultrastructural responses of the deprived myocardium to assess the utility of this in vitro model for studying myocardial necrosis, preservation, and repair. After 4 h of deprivation at 4, 24, 37, or 42 degrees C, 1) beating had ceased;2) ATP levels were decreased by 22% for 4 degrees C insults, 69% for 24 degrees C, 89% for 37 degrees C, and 97% for 42 degrees C; 3) CPK and LDH levels were unchanged; and 4) ultrastructural changes were observed. After 24 h of recovery from deprivation, 1) beating resumed, except for 42 degrees C;2) ATP levels were 102% of control for 4 degrees C; 99% for 24 degrees C; 62% for 37 degrees C; and 4% for 42 degrees C; 3) LDH content was decreased by 0% at 4 degrees C; 6% at 24 degrees C; 35% at 7 degrees C; and 70% at 42 degrees C; and 4) CPK content decreased similarly. Hypothermia protected deprived myocytes while hyperthermia accelerated cell necrosis. Combining deprivation with thermal insult in this in vitro model provides a spectrum of myocardial damage for studying the effect of interventions on repair processes and on metabolic changes in jeopardized myocardium.
Authors:
W R Roeske; J S Ingwall; M DeLuca; H D Sybers
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The American journal of physiology     Volume:  232     ISSN:  0002-9513     ISO Abbreviation:  Am. J. Physiol.     Publication Date:  1977 Mar 
Date Detail:
Created Date:  1977-04-30     Completed Date:  1977-04-30     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  0370511     Medline TA:  Am J Physiol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  H288-96     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Adenosine Triphosphate / metabolism
Animals
Creatine Kinase / metabolism
Female
Fetal Heart* / enzymology,  physiopathology,  ultrastructure
Glucose / metabolism
Hot Temperature*
L-Lactate Dehydrogenase / metabolism
Mice
Models, Biological
Necrosis / etiology*
Organ Culture Techniques
Oxygen Consumption
Pregnancy
Temperature
Tissue Preservation*
Chemical
Reg. No./Substance:
50-99-7/Glucose; 56-65-5/Adenosine Triphosphate; EC 1.1.1.27/L-Lactate Dehydrogenase; EC 2.7.3.2/Creatine Kinase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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