Document Detail


Thermally-induced cell lysis in Escherichia coli K12.
MedLine Citation:
PMID:  7738787     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Escherichia coli cells exposed to high temperatures exhibit a progressive loss of viability. We observed two mechanisms of cell death induced by lethal temperatures: with and without lysis. The number of cells lysed by heat decreased at later stages of the growth curve, when cells were pre-treated at lower temperatures for 10 minutes and when cells were pre-treated with novobiocin, nalidixic acid and cadmium chloride. Cell lysis was similar in wild type, rpoH, groE and dnaK mutant cells as well as in cells which overproduce heat shock proteins GroE or DnaK. Results using cells aligned for cell division and cells growing at 42 degrees C, 45 degrees C and 47 degrees C suggest that cells near division are more sensitive to lysis and that a high concentration of heat-shock proteins increases their resistance to lysis.
Authors:
J Membrillo-Hernández; A Núñez-de la Mora; T del Rio-Albrechtsen; R Camacho-Carranza; M C Gomez-Eichelmann
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of basic microbiology     Volume:  35     ISSN:  0233-111X     ISO Abbreviation:  J. Basic Microbiol.     Publication Date:  1995  
Date Detail:
Created Date:  1995-06-07     Completed Date:  1995-06-07     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  8503885     Medline TA:  J Basic Microbiol     Country:  GERMANY    
Other Details:
Languages:  eng     Pagination:  41-6     Citation Subset:  IM    
Affiliation:
Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, D.F., México.
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MeSH Terms
Descriptor/Qualifier:
Cadmium / pharmacology
Cadmium Chloride
Cell Division / drug effects
Chaperonin 10 / biosynthesis,  genetics
Chaperonin 60 / biosynthesis,  genetics
Chlorides / pharmacology
Escherichia coli / cytology*,  drug effects,  genetics
Escherichia coli Proteins*
Genes, Bacterial
HSP70 Heat-Shock Proteins / biosynthesis,  genetics
Hot Temperature
Mutation
Nalidixic Acid / pharmacology
Novobiocin / pharmacology
Chemical
Reg. No./Substance:
0/Chaperonin 10; 0/Chaperonin 60; 0/Chlorides; 0/Escherichia coli Proteins; 0/HSP70 Heat-Shock Proteins; 10108-64-2/Cadmium Chloride; 303-81-1/Novobiocin; 389-08-2/Nalidixic Acid; 7440-43-9/Cadmium; EC 3.6.1.-/dnaK protein, E coli

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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