Document Detail

Thermally responsive microcarriers with optimal poly(N-isopropylacrylamide) grafted density for facilitating cell adhesion/detachment in suspension culture.
MedLine Citation:
PMID:  22813847     Owner:  NLM     Status:  MEDLINE    
Large-scale cell culture of anchorage-dependent cells based on microcarriers is a crucial method for industrial-scale cell culture and large-scale expansion of therapeutic cells. Previously, the authors developed temperature-responsive microcarriers bearing poly(N-isopropylacrylamide) (PIPAAm)-grafted chains on their outer surface for the non-invasive detachment of cultured cells through temperature reduction without proteolytic enzyme treatment. In this study, to further facilitate cell adhesion and thermally induced detachment efficiency, PIPAAm-grafted beads with various grafted amounts and various grafted PIPAAm chain densities were prepared. Contact angle measurements at different temperatures revealed that the magnitude of the contact angle change from 37 to 20 °C decreased with increasing brush density. Additionally, the amount of fibronectin adsorbed on the bead surface decreased with increasing brush density. Chinese hamster ovary (CHO-K1) cells adhered to the surface of PIPAAm-grafted beads at 37 °C, and a negligible difference in the cell adhesive property was observed by varying the brush density of the PIPAAm-grafted beads. When the temperature was reduced to 20 °C, the adhering cells were found to detach themselves from the PIPAAm-grafted bead surfaces. Of particular interest, PIPAAm-grafted beads with intermediate brush density exhibited the highest efficiency of thermally induced cell detachment. Thus, the brush density of PIPAAm-grafted beads strongly affected the efficiency of thermally induced cell detachment.
Atsushi Tamura; Jun Kobayashi; Masayuki Yamato; Teruo Okano
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2012-07-17
Journal Detail:
Title:  Acta biomaterialia     Volume:  8     ISSN:  1878-7568     ISO Abbreviation:  Acta Biomater     Publication Date:  2012 Nov 
Date Detail:
Created Date:  2012-10-01     Completed Date:  2013-02-19     Revised Date:  2014-01-22    
Medline Journal Info:
Nlm Unique ID:  101233144     Medline TA:  Acta Biomater     Country:  England    
Other Details:
Languages:  eng     Pagination:  3904-13     Citation Subset:  IM    
Copyright Information:
Copyright © 2012 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.
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MeSH Terms
Acrylamides / chemical synthesis,  chemistry,  pharmacology*
Adsorption / drug effects
CHO Cells
Cell Adhesion / drug effects
Cell Culture Techniques / methods*
Fibronectins / metabolism
Microscopy, Phase-Contrast
Molecular Weight
Polymers / chemical synthesis,  chemistry,  pharmacology*
Surface Properties
Reg. No./Substance:
0/Acrylamides; 0/Fibronectins; 0/Polymers; 0/Suspensions; 25189-55-3/poly-N-isopropylacrylamide; 4R7X1O2820/Chlorine

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