| Thermally Responsive Microcarriers with Optimal Poly(N-isopropylacrylamide) Grafted Density for Facilitating Cell Adhesion/Detachment in Suspension Culture. | |
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MedLine Citation:
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PMID: 22813847 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Large-scale cell culture of anchorage-dependent cells based on microcarriers is a crucial method for industrial-scale cell culture and the large-scale expansion of therapeutic cells. Previously, we developed temperature-responsive microcarriers bearing poly(N-isopropylacrylamide) (PIPAAm)-grafted chains on their outer surface for the noninvasive detachment of cultured cells through temperature reduction without proteolytic enzyme treatment. In this study, to further facilitate cell adhesion and thermally induced detachment efficiency, PIPAAm-grafted beads with various grafted amounts and various grafted PIPAAm chain densities were prepared. Contact angle measurements at different temperatures revealed that the magnitude of the contact angle change from 37 to 20 °C decreased with increasing brush density. Additionally, the amount of fibronectin adsorbed onto the bead surface decreased with increasing brush density. Chinese hamster ovary (CHO-K1) cells adhered onto the surface of PIPAAm-grafted beads at 37 °C, and a negligible difference in the cell adhesive property was observed by varying the brush density of PIPAAm-grafted beads. After reducing the temperature to 20 °C, the adhering cells were found to detach themselves from the PIPAAm-grafted bead surfaces. Of particular interest, PIPAAm-grafted beads with intermediate brush density exhibited the highest efficiency of thermally induced cell detachment. Thus, the brush density of PIPAAm-grafted beads strongly affected the thermally induced cell detachment efficiency. |
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Authors:
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Atsushi Tamura; Jun Kobayashi; Masayuki Yamato; Teruo Okano |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2012-7-16 |
Journal Detail:
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Title: Acta biomaterialia Volume: - ISSN: 1878-7568 ISO Abbreviation: - Publication Date: 2012 Jul |
Date Detail:
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Created Date: 2012-7-20 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101233144 Medline TA: Acta Biomater Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
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Copyright © 2012. Published by Elsevier Ltd. |
Affiliation:
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Institute of Advanced Biomedical Engineering and Science, Tokyo Women's Medical University (TWIns), and Global Center of Excellence (COE), 8-1 Kawadacho, Shinjuku, Tokyo 162-8666, Japan. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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