Document Detail


Thermal stability and CD analysis of rat tyrosine hydroxylase.
MedLine Citation:
PMID:  7819204     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Tyrosine hydroxylase is the rate-limiting enzyme of catecholamine biosynthesis. It is a homotetramer made up of 56 kDa subunits. We examined the thermal stability of tyrosine hydroxylase purified from a rat pheochromocytoma cell line and investigated the relationship between enzyme activity and stability. Thermal stability was assessed by incubating the enzyme at an elevated temperature. Unfolding of the protein was followed by measuring the loss of circular dichroism (CD) at 220 nm. The CD loss was biphasic, with half-lives of 2 and 14 min at 55 degrees C in 100 mM potassium phosphate, pH 6.0. The rate of loss of enzyme activity paralleled the longer half-life under these conditions. This indicates that the structure of the active site is not appreciably change by the unfolding events corresponding to the first phase. Moreover, unfolding as assessed by the CD spectrum and activity was not reversible and did not exhibit a well-defined midpoint temperature or Tm. The thermal stability of the enzyme was altered by several factors that influence activity. The enzyme at pH 6.0 was less stable (t1/2 = 6.2 and 29 min) than the enzyme at pH 7.2 (a single t1/2 of 64 min). Phosphorylated tyrosine hydroxylase had shorter half-lives (t1/2 of 2 and 16 min) than the nonphosphorylated enzyme (t1/2 6.2 and 29 min) at pH 6.0, 50 degrees C, in 100 mM phosphate. Moderate changes in phosphate concentration had dramatic effects on enzyme stability. Decreasing the phosphate concentration from 50 to 10 mM (pH 6.0) increased the half-life from 2 and 23 min to greater than 120 min.(ABSTRACT TRUNCATED AT 250 WORDS)
Authors:
L G Gahn; R Roskoski
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Biochemistry     Volume:  34     ISSN:  0006-2960     ISO Abbreviation:  Biochemistry     Publication Date:  1995 Jan 
Date Detail:
Created Date:  1995-02-16     Completed Date:  1995-02-16     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0370623     Medline TA:  Biochemistry     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  252-6     Citation Subset:  IM    
Affiliation:
Department of Biochemistry and Molecular Biology, Louisiana State University Medical Center, New Orleans 70119.
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MeSH Terms
Descriptor/Qualifier:
Animals
Circular Dichroism
Enzyme Stability
Hydrogen-Ion Concentration
PC12 Cells
Phosphorylation
Polymers / pharmacology
Rats
Salts / pharmacology
Thermodynamics
Tyrosine 3-Monooxygenase / chemistry*
Grant Support
ID/Acronym/Agency:
NS-15994/NS/NINDS NIH HHS
Chemical
Reg. No./Substance:
0/Polymers; 0/Salts; 0/polyanions; EC 1.14.16.2/Tyrosine 3-Monooxygenase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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