| Thermal sensitivity of mitochondrial metabolism in two distinct mitotypes of Drosophila simulans: evaluation of mitochondrial plasticity. | |
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MedLine Citation:
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PMID: 20435817 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The overall aim of this study was to (1) evaluate the adaptive value of mitochondrial DNA by comparing mitochondrial performance in populations possessing different haplotypes and distribution, and to (2) evaluate the sensitivity of different enzymes of the electron transport system (ETS) during temperature-induced changes. We measured the impact of temperature of mitochondrial respiration and several key enzymes of mitochondrial metabolism in two mitotypes (siII and siIII) of Drosophila simulans. The temperature dependencies of oxygen consumption for mitochondria isolated from flight muscle was assessed with complex I substrates (pyruvate + malate + proline) and with sn glycerol-3-phosphate (to reduce complex III via glycerophosphate dehydrogenase) in both coupled and uncoupled states. Activities of citrate synthase, cytochrome c oxidase (COX), catalase and aconitase, and the excess capacity of COX at high convergent pathway flux were also measured as a function of temperature. Overall, our results showed that functional differences between the two mitotypes are few. Results suggest that differences between the two mitotypes could hardly explain the temperature-specific differences measured in mitochondria performances. It suggests that some other factor(s) may be driving the maintenance of mitotypes. We also show that the different enzymes of the ETS have different thermal sensitivities. The catalytic capacities of these enzymes vary with temperature changes, and the corresponding involvement of the different steps on mitochondrial regulation probably varies with temperature. For example, the excess COX capacity is low, even non-existent, at high and intermediate temperatures (18 degrees C, 24 degrees C and 28 degrees C) whereas it is quite high at a lower temperature (12 degrees C), suggesting release of respiration control by COX at low temperature. |
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Authors:
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Nicolas Pichaud; Etienne Hébert Chatelain; J William O Ballard; Robert Tanguay; Geneviève Morrow; Pierre U Blier |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: The Journal of experimental biology Volume: 213 ISSN: 1477-9145 ISO Abbreviation: J. Exp. Biol. Publication Date: 2010 May |
Date Detail:
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Created Date: 2010-05-03 Completed Date: 2010-08-02 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0243705 Medline TA: J Exp Biol Country: England |
Other Details:
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Languages: eng Pagination: 1665-75 Citation Subset: IM |
Affiliation:
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Laboratoire de biologie intégrative, Département de Biologie, Université du Québec à Rimouski, 300 Allée des Ursulines, Rimouski, Québec, Canada, G5L 3A1. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cell Respiration / drug effects Drosophila / drug effects, metabolism* Electron Transport Complex IV / antagonists & inhibitors, metabolism Kinetics Mitochondria / drug effects, enzymology, metabolism* Sodium Azide / toxicity Temperature* |
| Chemical | |
Reg. No./Substance:
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26628-22-8/Sodium Azide; EC 1.9.3.1/Electron Transport Complex IV |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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