| Thermal nociception in adult Drosophila: behavioral characterization and the role of the painless gene. | |
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MedLine Citation:
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PMID: 17081265 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Nociception, warning of injury that should be avoided, serves an important protective function in animals. In this study, we show that adult Drosophila avoids noxious heat by a jump response. To quantitatively analyze this nociceptive behavior, we developed two assays. In the CO2 laser beam assay, flies exhibit this behavior when a laser beam heats their abdomens. The consistency of the jump latency in this assay meets an important criterion for a good nociceptive assay. In the hot plate assay, flies jump quickly to escape from a hot copper plate (>45 degrees C). Our results demonstrate that, as in mammals, the latency of the jump response is inversely related to stimulus intensity, and innoxious thermosensation does not elicit this nociceptive behavior. To explore the genetic mechanisms of nociception, we examined several mutants in both assays. Abnormal nociceptive behavior of a mutant, painless, indicates that painless, a gene essential for nociception in Drosophila larvae, is also required for thermal nociception in adult flies. painless is expressed in certain neurons of the peripheral nervous system and thoracic ganglia, as well as in the definite brain structures, the mushroom bodies. However, chemical or genetic insults to the mushroom bodies do not influence the nociceptive behavior, suggesting that different painless-expressing neurons play diverse roles in thermal nociception. Additionally, no-bridge(KS49), a mutant that has a structural defect in the protocerebral bridge, shows defective response to noxious heat. Thus, our results validate adult Drosophila as a useful model to study the genetic mechanisms of thermal nociception. |
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Authors:
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S Y Xu; C L Cang; X F Liu; Y Q Peng; Y Z Ye; Z Q Zhao; A K Guo |
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Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't; Validation Studies |
Journal Detail:
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Title: Genes, brain, and behavior Volume: 5 ISSN: 1601-1848 ISO Abbreviation: Genes Brain Behav. Publication Date: 2006 Nov |
Date Detail:
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Created Date: 2006-11-03 Completed Date: 2006-12-22 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101129617 Medline TA: Genes Brain Behav Country: England |
Other Details:
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Languages: eng Pagination: 602-13 Citation Subset: IM |
Affiliation:
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State Key Laboratory of Brain and Cognitive Science, Institute of Biophysics, CAS, Graduate School of CAS, Beijing, China. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Avoidance Learning / physiology Drosophila Proteins / genetics*, metabolism Drosophila melanogaster / genetics*, metabolism Ganglia, Invertebrate / metabolism Ion Channels / genetics*, metabolism Mushroom Bodies / metabolism Pain / genetics* Pain Measurement / methods* Reaction Time / genetics Statistics, Nonparametric Temperature Sense / genetics* |
| Chemical | |
Reg. No./Substance:
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0/Drosophila Proteins; 0/Ion Channels; 0/painless protein, Drosophila |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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