Document Detail


Thermal and high-pressure inactivation kinetics of polyphenol oxidase in Victoria grape must.
MedLine Citation:
PMID:  15826049     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The inactivation kinetics of polyphenol oxidase (PPO) in freshly prepared grape must under high hydrostatic pressure (100-800 MPa) combined with moderate temperature (20-70 degrees C) was investigated. Atmospheric pressure conditions in a temperature range of 55-70 degrees C were also tested. Isothermal inactivation of PPO in grape must could be described by a biphasic model. The values of activation energy and activation volume of stable fraction were estimated as 53.34 kJ mol(-1) and -18.15 cm3 mol(-1) at a reference pressure of 600 MPa and reference temperature of 50 degrees C, respectively. Pressure and temperature were found to act synergistically, except in the high-temperature-low-pressure region where an antagonistic effect was found. A third-degree polynomial model was successfully applied to describe the temperature/pressure dependence of the inactivation rate constants of the stable PPO fraction in grape must.
Authors:
Gabriela Rapeanu; Ann Van Loey; Chantal Smout; Marc Hendrickx
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of agricultural and food chemistry     Volume:  53     ISSN:  0021-8561     ISO Abbreviation:  J. Agric. Food Chem.     Publication Date:  2005 Apr 
Date Detail:
Created Date:  2005-04-13     Completed Date:  2005-05-26     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  0374755     Medline TA:  J Agric Food Chem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  2988-94     Citation Subset:  IM    
Affiliation:
Faculty of Food Science and Engineering, Department of Bioengineering, University "Dunaren de Jos" Galati, Domneasca 111, 800201 Galati, Romania.
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MeSH Terms
Descriptor/Qualifier:
Catechol Oxidase / chemistry,  metabolism*
Enzyme Activation
Fruit / enzymology*
Hot Temperature*
Hydrostatic Pressure*
Kinetics
Thermodynamics
Vitis / enzymology*
Chemical
Reg. No./Substance:
EC 1.10.3.1/Catechol Oxidase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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