Document Detail

There is no complete linkage between the polymorphisms N680S and T307A of the follicular stimulating hormone receptor gene in fertile women.
MedLine Citation:
PMID:  21063764     Owner:  NLM     Status:  MEDLINE    
PURPOSE: We conducted a cross-sectional study to evaluate the linkage of FSHR T307A and N680S in a group of fertile women.
METHODS: Peripheral blood was obtained from 51 fertile women. DNA extraction and isolation were performed. For the detection of the T307A polymorphism a set of primers (5_-TCTGAGCTTCATCCAATTTGCA-3_and 5_-GGGAAAGAGGGCA GCTGCAA-3) was used and then the product was further amplified by a second PCR-RFLP using another set of primers (5_-CAAATCTATTTTAAGGCAAGAAGTTGATTATATGCCTCAG-3_and 5_-GTAGATTCCAATGCAGA GATCA-3). For the N680S polymorphism the primers (5_-TTTGTGGTCATCTGTGGCTGC-3_ and 5_-CAAAGGCAAGGACTGAATT ATC ATT-3_) were used. Statistical analysis for the association between the polymorphisms was performed by the Spearman test.
RESULTS: We calculated the association between the homozygosis at codon 307 and at codon 680 both for T/T-S/S and A/A-N/N. A significant association between the genotypic results at codon 680 with those at codon 307 was found (r = 0.6363, P = 0.001). However, a complete linkage between these two polymorphisms was rejected as there were 12 patients with discordant results from the expected A-N/T-S at codons 307 and 680, respectively.
CONCLUSION: The current data demonstrated an association but failed to demonstrate a complete linkage between these two polymorphisms.
Gustavo P Rodini; Vanessa K Genro; Ursula Matte; Fernanda S Pereira; João Paolo Bilibio; Camila Greggianin; Carlos A B Souza; João Sabino Cunha-Filho
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-11-10
Journal Detail:
Title:  Journal of assisted reproduction and genetics     Volume:  28     ISSN:  1573-7330     ISO Abbreviation:  J. Assist. Reprod. Genet.     Publication Date:  2011 Mar 
Date Detail:
Created Date:  2011-04-27     Completed Date:  2011-08-11     Revised Date:  2013-07-03    
Medline Journal Info:
Nlm Unique ID:  9206495     Medline TA:  J Assist Reprod Genet     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  221-4     Citation Subset:  IM    
Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil.
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MeSH Terms
Cross-Sectional Studies
DNA / isolation & purification
Fertility / genetics
Genetic Linkage*
Polymorphism, Restriction Fragment Length*
Receptors, FSH / genetics*
Sequence Analysis, DNA
Reg. No./Substance:
0/Receptors, FSH; 9007-49-2/DNA

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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