| Therapeutic efficacy of a tuberculosis DNA vaccine encoding heat shock protein 65 of Mycobacterium tuberculosis and the human interleukin 2 fusion gene. | |
| | |
MedLine Citation:
|
PMID: 19056317 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Use of therapeutic DNA vaccines is a promising strategy against tuberculosis (TB), however, their immunogenicity still needs to be improved. In this study, a plasmid DNA vaccine expressing heat shock protein 65 (HSP65) and the human interleukin 2 (IL-2) fusion gene was constructed. Immune responses induced by the vaccine in the mice and protection against Mycobacterium tuberculosis (MTB) were investigated, along with the therapeutic effect of the DNA vaccine on tuberculosis in mice. Administration of the HSP65-IL-2-DNA vaccine enhanced Th1-type cellular responses by producing greater amounts of interferon-gamma (IFN-gamma) and IL-2 with a higher titer of antigen-specific anti-Hsp65 IgG2a. Compared with the Bacille Calmette-Guérin (BCG) vaccine, the DNA vaccine was able to evoke both CD4 and CD8 T-cell responses, with an especially high percentage of CD8 T-cells. The DNA vaccine was also able to induce high antigen-specific cytotoxicity activity against target cells. When the mice were challenged with virulent MTB H37Rv, a dramatic decrease in the numbers of MTB colony forming units in the spleen and lungs was observed in the mice immunized with HSP65-IL-2-DNA (P<0.05). Meanwhile, the bacterial numbers in TB infected mice treated with the DNA vaccine were also significantly reduced. The protective and therapeutic effects of the HSP65-IL-2-DNA vaccine in the spleen and lungs were superior to that of the HSP65-DNA vaccine (P<0.05). These results suggest that the DNA vaccine expression of IL-2 and the HSP65 fusion gene enhances the immunogenicity and protective as well as therapeutic effects of the HSP65-DNA vaccine against TB in mice by improving the Th1-type response. |
| | |
Authors:
|
Shi Changhong; Zhang Hai; Wang Limei; An Jiaze; Xi Li; Zhang Tingfen; Xu Zhikai; Zhao Yong |
Related Documents
:
|
19523247 - Immune responses generated by intramuscular dna immunization of brugia malayi transglut... 11488667 - The effect of vaccination with dna encoding murine t-cell epitopes on the der p 1 and 2... 18300037 - Comparison of immune responses induced in mice by vaccination with dna vaccine construc... 18329757 - Enhancement of hiv dna vaccine immunogenicity by the nkt cell ligand, alpha-galactosylc... 8326127 - Candidate vaccine antigens that stimulate the cellular immune response of mice vaccinat... 2487447 - Anopheline species, some of their habits and relation to malaria in endemic areas of ro... |
Publication Detail:
|
Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't Date: 2008-12-03 |
Journal Detail:
|
Title: Tuberculosis (Edinburgh, Scotland) Volume: 89 ISSN: 1873-281X ISO Abbreviation: Tuberculosis (Edinb) Publication Date: 2009 Jan |
Date Detail:
|
Created Date: 2009-01-12 Completed Date: 2009-09-25 Revised Date: 2009-11-19 |
Medline Journal Info:
|
Nlm Unique ID: 100971555 Medline TA: Tuberculosis (Edinb) Country: Scotland |
Other Details:
|
Languages: eng Pagination: 54-61 Citation Subset: IM |
Affiliation:
|
Research Center of Laboratory Animals, Fourth Military Medical University, Xi'an, Shaanxi Province, China. changhong@fmmu.edu.cn |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Bacterial Proteins / genetics*, immunology CD4-Positive T-Lymphocytes / immunology CD8-Positive T-Lymphocytes / immunology Chaperonin 60 Chaperonins / genetics*, immunology Cytotoxicity Tests, Immunologic Humans Interleukin-2 / genetics*, immunology Lung / immunology, microbiology Male Mice Mice, Inbred BALB C Mycobacterium tuberculosis / immunology* Random Allocation Recombinant Fusion Proteins / genetics, immunology Spleen / immunology, microbiology Th1 Cells / immunology Th2 Cells / immunology Tuberculosis / immunology, prevention & control* Tuberculosis Vaccines / administration & dosage*, immunology Vaccines, DNA / administration & dosage*, immunology |
| Chemical | |
Reg. No./Substance:
|
0/Bacterial Proteins; 0/Chaperonin 60; 0/Interleukin-2; 0/Recombinant Fusion Proteins; 0/Tuberculosis Vaccines; 0/Vaccines, DNA; 0/heat-shock protein 65, Mycobacterium; EC 3.6.1.-/Chaperonins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Neutrophil extracellular traps are induced by Mycobacterium tuberculosis.
Next Document: Potential challenges to the Stop TB Plan for humans in China; cattle maintain M. bovis and M. tuberc...