Document Detail

Therapeutic angiogenesis induced by local autologous bone marrow cell implantation.
MedLine Citation:
PMID:  11996265     Owner:  NLM     Status:  MEDLINE    
BACKGROUND: Therapeutic angiogenesis was induced by local autologous bone marrow cell implantation (BMCI) in ischemic hindlimb or ischemic heart models in rats. This study was designed to investigate the toxicity and therapeutic potency of local BMCI using a chronic coronary occlusion model in dogs. METHODS: The canine chronic coronary occlusion model was created by ligating of the left anterior descending artery (LAD). The myocardium in the left ventricle was divided into distinct normal, marginal, and infarction areas 30 days after LAD ligation. Each area was injected at two locations, with either 2 x 10(7) bone marrow cells (n = 7, BMCI group) or 0.1 mL phosphate-buffered saline (PBS) only (n = 7, PBS group), respectively. Hemodynamics were evaluated by a single ultrasonic transducer and echocardiography before and 30 days after the treatment. Angiogenesis was evaluated by vessel count 30 days after the treatment. The toxicity of BMCI treatment was also evaluated in 8 normal dogs by following changes in electrocardiography (ECG), echocardiography, local histology, and systemic biochemistry indexes. RESULTS: There was a significantly higher percentage of wall thickening in the marginal area in the BMCI group than in the PBS group 30 days after treatment (14.5 +/- 2.28 versus 8.1 +/- 3.00, p = 0.002). Significantly more microvessels were observed in the marginal area in the BMCI group than in the PBS group 30 days after treatment (127.7 +/- 20.1 versus 88.0 +/- 10.2/field, p = 0.0007). No systemic or local toxicity was found following BMCI treatment in the acute or chronic phases. CONCLUSIONS: BMCI treatment improved local wall thickening dynamics, presumably due to the angiogenesis induced by the treatment. This indicates that it might be a safe and effective therapy for ischemic heart disease.
Kimikazu Hamano; Tao-Sheng Li; Toshiro Kobayashi; Ken Hirata; Masafumi Yano; Michihiro Kohno; Masunori Matsuzaki
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Annals of thoracic surgery     Volume:  73     ISSN:  0003-4975     ISO Abbreviation:  Ann. Thorac. Surg.     Publication Date:  2002 Apr 
Date Detail:
Created Date:  2002-05-08     Completed Date:  2002-05-21     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  15030100R     Medline TA:  Ann Thorac Surg     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1210-5     Citation Subset:  AIM; IM    
First Department of Surgery, Yamaguchi University School of Medicine, Ube, Japan.
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MeSH Terms
Bone Marrow Transplantation*
Cell Transplantation / adverse effects
Coronary Vessels / growth & development*,  pathology
Microcirculation / pathology
Myocardial Contraction
Myocardial Ischemia / pathology,  physiopathology,  therapy*,  ultrasonography
Neovascularization, Physiologic*
Transplantation, Autologous
Ventricular Function, Left

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