Document Detail

Theoretical study of the partial molar volume change associated with the pressure-induced structural transition of ubiquitin.
MedLine Citation:
PMID:  17660257     Owner:  NLM     Status:  MEDLINE    
The partial molar volume (PMV) change associated with the pressure-induced structural transition of ubiquitin is analyzed by the three-dimensional reference interaction site model (3D-RISM) theory of molecular solvation. The theory predicts that the PMV decreases upon the structural transition, which is consistent with the experimental observation. The volume decomposition analysis demonstrates that the PMV reduction is primarily caused by the decrease in the volume of structural voids in the protein, which is partially canceled by the volume expansion due to the hydration effects. It is found from further analysis that the PMV reduction is ascribed substantially to the penetration of water molecules into a specific part of the protein. Based on the thermodynamic relation, this result implies that the water penetration causes the pressure-induced structural transition. It supports the water penetration model of pressure denaturation of proteins proposed earlier.
Takashi Imai; Shusaku Ohyama; Andriy Kovalenko; Fumio Hirata
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2007-07-27
Journal Detail:
Title:  Protein science : a publication of the Protein Society     Volume:  16     ISSN:  0961-8368     ISO Abbreviation:  Protein Sci.     Publication Date:  2007 Sep 
Date Detail:
Created Date:  2007-09-03     Completed Date:  2007-12-11     Revised Date:  2013-06-06    
Medline Journal Info:
Nlm Unique ID:  9211750     Medline TA:  Protein Sci     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1927-33     Citation Subset:  IM    
Department of Bioscience and Bioinformatics, Ritsumeikan University, Kusatsu, Shiga 525-8577, Japan.
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MeSH Terms
Hydrostatic Pressure
Models, Molecular
Models, Theoretical*
Protein Conformation
Protein Denaturation
Ubiquitin / chemistry*
Water / chemistry
Reg. No./Substance:
0/Ubiquitin; 7732-18-5/Water

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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