| Theoretical analysis of a site-specific chemiluminescence reaction and its application to quantitation of lipid hydroperoxides. | |
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MedLine Citation:
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PMID: 3242948 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A system was designed for chemiluminescent measurement of lipid hydroperoxides by their site-specific reaction in sodium dodecylsulfate micelles. Ferrous ion-induced decomposition of lipid hydroperoxides in the sodium dodecylsulfate micelles resulted in strong chemiluminescence of the Cypridina luciferin analog, 2-methyl-6-phenyl-3,7-dihydroimidazo[1,2-alpha]pyrazin-3-one (CLA). After addition of ferrous sulfate to the micelles containing lipid hydroperoxide and luciferin, the chemiluminescence intensity reached a maximum rapidly and then decreased. The sequence of this reaction was elucidated by theoretical analysis, which demonstrated that the maximum chemiluminescence intensity is proportional to the initial concentration of hydroperoxide. Good linear relationships were observed between the maximum counts of chemiluminescence and the amounts of hydroperoxides of linoleic acid, phosphatidylcholine, choresterol (5 alpha), cumene and tert-butyl and hydrogen peroxide. This chemiluminescence method was simple and sensitive enough to detect picomole levels of linoleic acid and phosphatidylcholine hydroperoxides. |
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Authors:
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K Fukuzawa; T Yoshimura; T Fujii; K Takauchi; M Miki; H Tamai; M Mino |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Chemistry and physics of lipids Volume: 48 ISSN: 0009-3084 ISO Abbreviation: Chem. Phys. Lipids Publication Date: 1988 Oct |
Date Detail:
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Created Date: 1989-05-26 Completed Date: 1989-05-26 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0067206 Medline TA: Chem Phys Lipids Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 197-203 Citation Subset: IM |
Affiliation:
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Faculty of Pharmaceutical Sciences, University of Tokushima, Japan. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Binding Sites Ferrous Compounds / metabolism Lipid Peroxides / analysis* Luminescent Measurements* Micelles Oxidation-Reduction |
| Chemical | |
Reg. No./Substance:
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0/Ferrous Compounds; 0/Lipid Peroxides; 0/Micelles |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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