Document Detail


Theoretical and Experimental Analysis of Electroporated Membrane Conductance in Cell Suspension.
MedLine Citation:
PMID:  21193368     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
An intense electric field can be applied to increase the membrane conductance (Gm) and consequently, the conductivity of cell suspension. This phenomenon is called electroporation. This mechanism is used in a wide range of medical applications, genetic engineering, and therapies. Conductivity measurements of cell suspensions were carried out during application of electric fields from 40 kV/m to 165 kV/m. Experimental results were analyzed with two electroporation models: the asymptotic electroporation model was used to estimate Gm at the beginning and at the end of electric field pulse, and the extended Kinosita electroporation model to increase Gm linearly in time. The maximum Gm was 1-7e104 S/m2, and the critical angle (when the Gm is insignificant) was 50 to 65 degrees. In addition, the sensitivity of electroporated membrane conductance to extracellular and cytoplasmatic conductivity and cell radius has been studied. This study showed that external conductivity and cell radius are important parameters affecting the pore-opening phenomenon. However, if the cell radius is larger than 7 Ým in low conductivity medium, the cell dimensions are not so important.
Authors:
D O H Suzuki; A Ramos; M C M Ribeiro; L H Cazarolli; F R M B Silva; L D Leite; J L B Marques
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2010-12-30
Journal Detail:
Title:  IEEE transactions on bio-medical engineering     Volume:  -     ISSN:  1558-2531     ISO Abbreviation:  -     Publication Date:  2010 Dec 
Date Detail:
Created Date:  2011-1-3     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0012737     Medline TA:  IEEE Trans Biomed Eng     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
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