Document Detail


Thematic review series: glycerolipids. Mammalian glycerol-3-phosphate acyltransferases: new genes for an old activity.
MedLine Citation:
PMID:  18658143     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Glycerol-3-phosphate acyltransferases (GPATs; EC2.3.1.15) catalyze the first step in the de novo synthesis of neutral lipids (triglycerides) and glycerophospholipids. The existence of multiple enzyme isoforms with GPAT activity was predicted many years ago when GPAT activities with distinct kinetic profiles and sensitivity to inhibitors were characterized in two subcellular compartments, mitochondria and microsomes. We now know that mammals have at least four GPAT isoforms with distinct tissue distribution and function. GPAT1 is the major mitochondrial GPAT isoform and is characterized by its resistance to sulfhydryl-modifying reagents, such as N-ethylmaleimide (NEM). GPAT2 is a minor NEM-sensitive mitochondrial isoform. The activity referred to as microsomal GPAT is encoded by two closely related genes, GPAT3 and GPAT4. GPAT isoforms are important regulators of cellular triglyceride and phospholipid content, and may channel fatty acids toward particular metabolic fates. Overexpression and knock-out studies suggest that GPAT isoforms can play important roles in the development of hepatic steatosis, insulin resistance, and obesity; GPAT isoforms are also important for lactation. This review summarizes the current state of knowledge on mammalian GPAT isoforms.
Authors:
Ruth E Gimeno; Jingsong Cao
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Publication Detail:
Type:  Journal Article; Review     Date:  2008-07-24
Journal Detail:
Title:  Journal of lipid research     Volume:  49     ISSN:  0022-2275     ISO Abbreviation:  J. Lipid Res.     Publication Date:  2008 Oct 
Date Detail:
Created Date:  2008-09-15     Completed Date:  2008-10-22     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0376606     Medline TA:  J Lipid Res     Country:  United States    
Other Details:
Languages:  eng     Pagination:  2079-88     Citation Subset:  IM    
Affiliation:
Cardiovascular and Metabolic Diseases, Wyeth Research, Cambridge, MA 02140, USA. rgimeno@wyeth.com
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Motifs
Amino Acid Sequence
Animals
Ethylmaleimide / metabolism
Glycerol-3-Phosphate O-Acyltransferase / chemistry,  genetics*,  metabolism*
Humans
Isoenzymes / chemistry,  genetics,  metabolism
Microsomes / enzymology,  metabolism
Chemical
Reg. No./Substance:
0/Isoenzymes; 128-53-0/Ethylmaleimide; EC 2.3.1.15/Glycerol-3-Phosphate O-Acyltransferase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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