Document Detail


Theaflavins from black tea, especially theaflavin-3-gallate, reduce the incorporation of cholesterol into mixed micelles.
MedLine Citation:
PMID:  19049290     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Tea is one of the most widely consumed beverages in the world and may be associated with reduced heart disease rates. Theaflavins, which are formed in the production of black tea, have been suggested being responsible for the blood-cholesterol-lowering (BCL) effects of tea. We hypothesized that the effect of theaflavins on BCL could be through interference in the formation of dietary mixed micelles, which could result in reduced intestinal cholesterol absorption. Micelles were produced by mixing oleic acid, bile acids, lyso-phosphatidylcholine, and cholesterol. Theaflavin-treated micelles/particles were analyzed using electron microscopy (cryo-TEM), high-performance liquid chromatography (HPLC) analysis, and light-scattering particle size measurements. A dose-dependent inhibitory effect of theaflavins on the incorporation of (14)C-labeled cholesterol into micelles and a theaflavin-dependent increase in particle size was found. These particles consisted of insoluble large multilamellar vesicles with onion-like structures. Ultracentrifugation and HPLC analysis revealed that the pellets contained mainly theaflavin-3-gallate, while the remaining theaflavins were found to be present in the supernatant. Using purified theaflavin subtypes confirmed that mainly theaflavin-3-gallate is responsible for multilamellar vesicle formation. These results show that theaflavins can play a role in decreased intestinal cholesterol absorption via inhibition of micelle formation.
Authors:
Mario A Vermeer; Theo P J Mulder; Henri O F Molhuizen
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of agricultural and food chemistry     Volume:  56     ISSN:  1520-5118     ISO Abbreviation:  J. Agric. Food Chem.     Publication Date:  2008 Dec 
Date Detail:
Created Date:  2008-12-18     Completed Date:  2009-01-26     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0374755     Medline TA:  J Agric Food Chem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  12031-6     Citation Subset:  IM    
Affiliation:
Unilever Food and Health Research Institute, Olivier van Noortlaan 120, P. O. Box 114, 3130 AC, 3133 AT Vlaardingen, The Netherlands. mario.vermeer@unilever.com
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MeSH Terms
Descriptor/Qualifier:
Biflavonoids / chemistry*,  pharmacology
Catechin / chemistry*,  pharmacology
Cholesterol / chemistry*,  metabolism
Gallic Acid / analogs & derivatives*,  chemistry,  pharmacology
Humans
Intestinal Absorption / drug effects
Micelles*
Microscopy, Electron, Transmission
Plant Extracts / chemistry*,  pharmacology
Polymers / chemistry*
Tea / chemistry*
Chemical
Reg. No./Substance:
0/Biflavonoids; 0/Micelles; 0/Plant Extracts; 0/Polymers; 0/Tea; 149-91-7/Gallic Acid; 154-23-4/Catechin; 31629-79-5/theaflavine gallate; 57-88-5/Cholesterol

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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