Document Detail


Th17 cells induce colitis and promote Th1 cell responses through IL-17 induction of innate IL-12 and IL-23 production.
MedLine Citation:
PMID:  21531892     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Both Th1 and Th17 cells have been implicated in the pathogenesis of inflammatory bowel disease and experimental colitis. However, the complex relationship between Th1 and Th17 cells and their relative contributions to the pathogenesis of inflammatory bowel disease have not been completely analyzed. Although it has been recently shown that Th17 cells can convert into Th1 cells, the underlying in vivo mechanisms and the role of Th1 cells converted from Th17 cells in the pathogenesis of colitis are still largely unknown. In this study, we report that Th17 cells from CBir1 TCR transgenic mice, which are specific for an immunodominant microbiota Ag, are more potent than Th1 cells in the induction of colitis, as Th17 cells induced severe colitis, whereas Th1 cells induced mild colitis when transferred into TCRβxδ(-/-) mice. High levels of IL-12 and IL-23 and substantial numbers of IFN-γ(+) Th1 cells emerged in the colons of Th17 cell recipients. Administration of anti-IL-17 mAb abrogated Th17 cell-induced colitis development, blocked colonic IL-12 and IL-23 production, and inhibited IFN-γ(+) Th1 cell induction. IL-17 promoted dendritic cell production of IL-12 and IL-23. Furthermore, conditioned media from colonic tissues of colitic Th17 cell recipients induced IFN-γ production by Th17 cells, which was inhibited by blockade of IL-12 and IL-23. Collectively, these data indicate that Th17 cells convert to Th1 cells through IL-17 induction of mucosal innate IL-12 and IL-23 production.
Authors:
Ting Feng; Hongwei Qin; Lanfang Wang; Etty N Benveniste; Charles O Elson; Yingzi Cong
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2011-04-29
Journal Detail:
Title:  Journal of immunology (Baltimore, Md. : 1950)     Volume:  186     ISSN:  1550-6606     ISO Abbreviation:  J. Immunol.     Publication Date:  2011 Jun 
Date Detail:
Created Date:  2011-05-20     Completed Date:  2011-08-11     Revised Date:  2013-06-30    
Medline Journal Info:
Nlm Unique ID:  2985117R     Medline TA:  J Immunol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  6313-8     Citation Subset:  AIM; IM    
Affiliation:
Department of Microbiology and Immunology, University of Texas Medical Branch, Galveston, TX 77555-1019, USA.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Animals
Antibodies, Monoclonal / immunology,  pharmacology
Cell Differentiation / immunology
Cells, Cultured
Colitis / immunology*,  metabolism,  pathology
Colon / drug effects,  immunology,  metabolism
Culture Media, Conditioned / pharmacology
Female
Flagellin / genetics,  immunology
Flow Cytometry
Interferon-gamma / immunology,  metabolism
Interleukin-12 / immunology*,  metabolism
Interleukin-17 / immunology*,  metabolism
Interleukin-23 / immunology*,  metabolism
Intestines / immunology,  metabolism,  pathology
Male
Mice
Mice, Inbred C57BL
Mice, Knockout
Mice, Transgenic
Organ Culture Techniques
Receptors, Antigen, T-Cell / genetics,  immunology
T-Lymphocytes / immunology,  metabolism
Th1 Cells / immunology*,  metabolism
Th17 Cells / drug effects,  immunology*,  metabolism
Grant Support
ID/Acronym/Agency:
AI083484/AI/NIAID NIH HHS; C06 RR020136/RR/NCRR NIH HHS; DK064400/DK/NIDDK NIH HHS; DK071176/DK/NIDDK NIH HHS; DK079918/DK/NIDDK NIH HHS; NS50655/NS/NINDS NIH HHS; NS57563/NS/NINDS NIH HHS; P01 DK071176/DK/NIDDK NIH HHS; R01 DK079918-05/DK/NIDDK NIH HHS; R21 AI083484-03/AI/NIAID NIH HHS; R24 DK064400/DK/NIDDK NIH HHS; RR-20136/RR/NCRR NIH HHS
Chemical
Reg. No./Substance:
0/Antibodies, Monoclonal; 0/CBir1 flagellin; 0/Culture Media, Conditioned; 0/Interleukin-17; 0/Interleukin-23; 0/Receptors, Antigen, T-Cell; 12777-81-0/Flagellin; 187348-17-0/Interleukin-12; 82115-62-6/Interferon-gamma
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Upregulation of IL-21 receptor on B cells and IL-21 secretion distinguishes novel 2009 H1N1 vaccine ...
Next Document:  Donor CD8 T cells and IFN-gamma are critical for sex-based differences in donor CD4 T cell engraftme...