| Tgf-beta-mediated FasL-Fas-Caspase pathway is crucial during palatogenesis. | |
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MedLine Citation:
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PMID: 21593251 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Programmed cell death, or apoptosis, is one of the fates of the medial edge epithelium (MEE) during palatal fusion. Transforming growth factor β (Tgf-β) signaling (such as Tgf-β3) is required for the disappearance of the MEE, but the relationship between Tgf-β3 and apoptosis remains unclear. Here we show that the Fas ligand (FasL)-Fas-Caspase extrinsic apoptosis pathway functions during palatal fusion in wild-type mice, but is not detectable in mice lacking Tgf-β3 (Tgf-β3 (-/-) ) or Tgfβr2 in the MEE (K14-Cre;Tgfbr2 (fl/fl)). Inhibition of the FasL-Fas system results in persistence of the midline epithelial seam (MES) and inhibition of caspase activity during palatal organ culture. Moreover, ectopic FasL protein induces apoptosis in MES of K14-Cre;Tgfbr2 (fl/fl) mice. Thus, we conclude that the FasL-Fas-caspase extrinsic apoptosis pathway is regulated by the Tgf-β3 signaling cascade and is essential for palatal fusion during craniofacial development. |
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Authors:
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X Huang; T Yokota; J Iwata; Y Chai |
Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't Date: 2011-05-18 |
Journal Detail:
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Title: Journal of dental research Volume: 90 ISSN: 1544-0591 ISO Abbreviation: J. Dent. Res. Publication Date: 2011 Aug |
Date Detail:
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Created Date: 2011-07-13 Completed Date: 2011-09-12 Revised Date: 2011-09-14 |
Medline Journal Info:
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Nlm Unique ID: 0354343 Medline TA: J Dent Res Country: United States |
Other Details:
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Languages: eng Pagination: 981-7 Citation Subset: D; IM |
Affiliation:
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Department of Stomatology, Beijing Friendship Hospital, Capital Medical University, Beijing 100050, China. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Antigens, CD95 / metabolism* Apoptosis / physiology* Caspase 3 / metabolism Caspase 8 / metabolism Caspases / metabolism* Cleft Palate / embryology Epithelial Cells / metabolism Epithelium / embryology Fas Ligand Protein / metabolism* Gene Expression Regulation, Developmental Mice Mice, Knockout Palate, Hard / embryology* Signal Transduction Transforming Growth Factor beta3 / physiology* |
| Grant Support | |
ID/Acronym/Agency:
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R01 DE014078/DE/NIDCR NIH HHS; R37 DE012711/DE/NIDCR NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Antigens, CD95; 0/Fas Ligand Protein; 0/Fas protein, mouse; 0/Fasl protein, mouse; 0/Transforming Growth Factor beta3; EC 3.4.22.-/Caspase 3; EC 3.4.22.-/Caspase 8; EC 3.4.22.-/Caspases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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