Document Detail

Tetraspanins in intercellular adhesion of polarized epithelial cells: spatial and functional relationship to integrins and cadherins.
MedLine Citation:
PMID:  11171326     Owner:  NLM     Status:  MEDLINE    
The subcellular distribution of tetraspanin molecules and their functional relationship with integrins in cell-cell adhesion was studied in detail in different polarized epithelial cell models. CD9, CD81 and CD151 tetraspanins were localized at lateral cell-cell contact sites in a similar distribution to E-cadherin. Interestingly, CD9 was partially localized at the apical microvillae of Madin-Darby canine kidney cells forming multimolecular complexes distinct from those found on the basolateral membrane, suggesting the coexistence of differential tetraspanin webs with different subcellular localization. We found that tetraspanin-associated beta1 integrins at cell-to-cell contacts were in a low-affinity conformational state, and that their localization at intercellular contacts was independent of cadherin expression and adhesion. Furthermore, integrin-tetraspanin complexes were functionally relevant in cell-cell adhesion in a cadherin-independent manner, without requiring a conformational change of the integrin moiety. Nevertheless, the integrin alpha3beta1 was ligand-binding competent and this binding did not disrupt association to tetraspanins. Moreover, Chinese hamster ovary cells treated with anti-tetraspanin mAbs or activatory anti-beta1 integrin mAbs were able to develop tubule-like structures. Together, these data support tetraspanin association as a new regulatory mechanism of integrin function and suggest a role for tetraspanins-integrin complexes in providing the cell with the spatial cues necessary for their proper polarization.
M Yáñez-Mó; R Tejedor; P Rousselle; F Sánchez -Madrid
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of cell science     Volume:  114     ISSN:  0021-9533     ISO Abbreviation:  J. Cell. Sci.     Publication Date:  2001 Feb 
Date Detail:
Created Date:  2001-02-22     Completed Date:  2001-05-03     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0052457     Medline TA:  J Cell Sci     Country:  England    
Other Details:
Languages:  eng     Pagination:  577-87     Citation Subset:  IM    
Servicio de Inmunología, Hospital Universitario de la Princesa, Universidad Autónoma de Madrid, 28006 Madrid, Spain.
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MeSH Terms
Antigens, CD / metabolism
Cadherins / metabolism,  physiology*
Cell Adhesion*
Cell Line
Cell Membrane / metabolism
Chromatography, Affinity
Epithelial Cells / cytology
Flow Cytometry
Fluorescent Antibody Technique
Integrins / metabolism,  physiology*
Membrane Proteins / metabolism,  physiology*
Microscopy, Confocal
Protein Binding
Reg. No./Substance:
0/Antigens, CD; 0/Cadherins; 0/Integrins; 0/Membrane Proteins

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