Document Detail

Tetraploid embryos rescue the early defects of tw5/tw5 mouse embryos.
MedLine Citation:
PMID:  14666509     Owner:  NLM     Status:  MEDLINE    
tclw5 is a t-complex recessive lethal mutation of the tw5-haplotype. Since tw5/tw5 embryos die soon after implantation, the tclw5 gene is thought to play an important role in early embryogenesis. Previous histological studies have demonstrated that tw5 homozygotes do not survive past the gastrulation stage due to extensive death of the embryonic ectoderm, whereas the extraembryonic tissues were less affected. In the present study, we demonstrate that tw5/tw5 embryos may be distinguished from wildtype littermates at embryonic (E) day 5.5. At this stage, the visceral endoderm of tw5/tw5 embryos appeared to be different, possessing smaller and fewer vacuoles compared to normal littermates. This led us to hypothesize that the visceral endoderm may be affected by tclw5. Confirmation was provided by the rescue of tw5/tw5 embryos following aggregation with tetraploid embryos. However, rescued embryos did not survive past E9.0 and displayed an underdeveloped posterior region. This would indicate that the actions of tclw5 extend beyond the midgestation stage.
Michihiko Sugimoto; Yuko Karashima; Kuniya Abe; Seong-Seng Tan; Nobuo Takagi
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Genesis (New York, N.Y. : 2000)     Volume:  37     ISSN:  1526-954X     ISO Abbreviation:  Genesis     Publication Date:  2003 Dec 
Date Detail:
Created Date:  2003-12-10     Completed Date:  2004-08-25     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  100931242     Medline TA:  Genesis     Country:  United States    
Other Details:
Languages:  eng     Pagination:  162-71     Citation Subset:  IM    
Copyright Information:
Copyright 2003 Wiley-Liss, Inc.
Division of Bioscience, Graduate School of Environmental Earth Science, Hokkaido University, Sapporo, Japan.
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MeSH Terms
Embryo, Mammalian / cytology
Embryonic and Fetal Development / genetics*
Endoderm / cytology*
Gastrula / cytology*
Genes, Lethal
Genes, Recessive*
Genomic Imprinting
Mice, Inbred C57BL

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