| Tetranectin immunoreactivity in normal human tissues. An immunohistochemical study of exocrine epithelia and mesenchyme. | |
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MedLine Citation:
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PMID: 2670845 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A recently discovered human plasma protein, tetranectin (TN), which has previously been demonstrated immunohistochemically within various endocrine tissues, was in this study identified in an additional number of epithelial and mesenchymal cells by two polyclonal antibodies and one monoclonal using the conventional immunoperoxidase staining technique and a modification of the CLONO-GLAD procedure. TN was found in endothelial and epithelial tissues, particularly in cells with a high turn-over or storage function such as gastric parietal and zymogenic cells, absorptive surface epithelium of the small intestine, ducts of exocrine glands and pseudostratified respiratory epithelium. Also mesenchymal cells produced a TN positive staining reaction, which was most conspicuous in mast cells, but also present in some lymphocytes, plasma cells, macrophages, granulocytes, striated and smooth muscle cells and fibroblasts. SDS-PAGE and Western blotting analysis of cultured human embryonal fibroblasts (WI-38) showed that the cells besides TN contain another protein with a molecular weight of 82,000. As this protein, however, reacted with our affinity purified antibodies it probably represents a precursor of TN or a protein with a molecular weight of approximately 60,000, which is covalently linked to TN. This and the fact that TN shows amino acid sequence homologies to the carboxyterminal part of the asialo-glycoprotein receptor and a cartilage proteoglycan core protein as well a binding affinity to plasminogen points to TN as being part of a larger molecule, which possibly has been cleaved by proteolysis at the cellular site and then passed into the blood, where it polymerizes into a tetramer. |
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Authors:
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L Christensen; I Clemmensen |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Histochemistry Volume: 92 ISSN: 0301-5564 ISO Abbreviation: Histochemistry Publication Date: 1989 |
Date Detail:
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Created Date: 1989-10-10 Completed Date: 1989-10-10 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0411300 Medline TA: Histochemistry Country: GERMANY, WEST |
Other Details:
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Languages: eng Pagination: 29-35 Citation Subset: IM |
Affiliation:
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Department of Pathology, Rigshospitalet, Copenhagen, Denmark. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Antibodies Antibodies, Monoclonal Blood Proteins / analysis*, immunology Blotting, Western Epithelium / analysis* Exocrine Glands / analysis* Humans Immunoenzyme Techniques Immunohistochemistry Lectins, C-Type* Mesoderm / analysis* |
| Chemical | |
Reg. No./Substance:
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0/Antibodies; 0/Antibodies, Monoclonal; 0/Blood Proteins; 0/Lectins, C-Type; 109489-77-2/tetranectin |
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