Document Detail


Tetranectin immunoreactivity in normal human tissues. An immunohistochemical study of exocrine epithelia and mesenchyme.
MedLine Citation:
PMID:  2670845     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A recently discovered human plasma protein, tetranectin (TN), which has previously been demonstrated immunohistochemically within various endocrine tissues, was in this study identified in an additional number of epithelial and mesenchymal cells by two polyclonal antibodies and one monoclonal using the conventional immunoperoxidase staining technique and a modification of the CLONO-GLAD procedure. TN was found in endothelial and epithelial tissues, particularly in cells with a high turn-over or storage function such as gastric parietal and zymogenic cells, absorptive surface epithelium of the small intestine, ducts of exocrine glands and pseudostratified respiratory epithelium. Also mesenchymal cells produced a TN positive staining reaction, which was most conspicuous in mast cells, but also present in some lymphocytes, plasma cells, macrophages, granulocytes, striated and smooth muscle cells and fibroblasts. SDS-PAGE and Western blotting analysis of cultured human embryonal fibroblasts (WI-38) showed that the cells besides TN contain another protein with a molecular weight of 82,000. As this protein, however, reacted with our affinity purified antibodies it probably represents a precursor of TN or a protein with a molecular weight of approximately 60,000, which is covalently linked to TN. This and the fact that TN shows amino acid sequence homologies to the carboxyterminal part of the asialo-glycoprotein receptor and a cartilage proteoglycan core protein as well a binding affinity to plasminogen points to TN as being part of a larger molecule, which possibly has been cleaved by proteolysis at the cellular site and then passed into the blood, where it polymerizes into a tetramer.
Authors:
L Christensen; I Clemmensen
Related Documents :
8338235 - Dynamics of epithelial cells in the corpus of the mouse stomach. iv. bidirectional migr...
2441515 - Human chorionic gonadotropin in gastric carcinoma. an immunohistochemical study suggest...
170785 - The relationship of gastrointestinal endocrine cells to gastric epithelial changes with...
15153415 - The "normal" endocrine cell of the gut: changing concepts and new evidences.
8866835 - Effects of rebamipide on gastric cell damage by helicobacter pylori-stimulated human ne...
10972975 - Down-regulation of n-acetylglucosaminyltransferase v by tumorigenesis- or metastasis-su...
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Histochemistry     Volume:  92     ISSN:  0301-5564     ISO Abbreviation:  Histochemistry     Publication Date:  1989  
Date Detail:
Created Date:  1989-10-10     Completed Date:  1989-10-10     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0411300     Medline TA:  Histochemistry     Country:  GERMANY, WEST    
Other Details:
Languages:  eng     Pagination:  29-35     Citation Subset:  IM    
Affiliation:
Department of Pathology, Rigshospitalet, Copenhagen, Denmark.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Antibodies
Antibodies, Monoclonal
Blood Proteins / analysis*,  immunology
Blotting, Western
Epithelium / analysis*
Exocrine Glands / analysis*
Humans
Immunoenzyme Techniques
Immunohistochemistry
Lectins, C-Type*
Mesoderm / analysis*
Chemical
Reg. No./Substance:
0/Antibodies; 0/Antibodies, Monoclonal; 0/Blood Proteins; 0/Lectins, C-Type; 109489-77-2/tetranectin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Postnatal development of glial fibrillary acidic protein (GFAP) immunoreactivity in pituicytes and t...
Next Document:  Localization of fibronectin in the folliculo-stellate cells of the rat anterior pituitary by the dou...