Document Detail

Tetrahydroisoquinolinecarboxylic acids and catecholamine metabolism in adrenal medulla explants.
MedLine Citation:
PMID:  7150353     Owner:  NLM     Status:  MEDLINE    
In a study of the relationship of the tetrahydroisoquinolinecarboxylic acids (TIQCAs) to catecholamine metabolism, we have investigated their effects on cultured rat adrenal medulla explants. Medullae were incubated in medium containing norlaudanosolinecarboxylic acid (NLCA) or 3',4'-deoxynorlaudanosolinecarboxylic acid (DNLCA) (0.5 mM) in the presence and absence of [3H]tyrosine. By paired-ion reverse-phase high pressure liquid chromatography, tissue epinephrine (EPI), norepinephrine (NE), dopamine (DA) and TIQCA were resolved. Endogenous concentrations were measured with electrochemical detection, and radioactivity was assayed by collecting appropriate effluents. Tissue levels of the TIQCAs reached saturating levels of 0.36 mM by about 20 hr. DNLCA elicited a significant decrease (60%) in endogenous DA, NE and EPI at 40 hr, whereas only DA was depressed at 30 hr. NLCA had little effect after 30 or 40 hr. When tissues were maintained in the presence of alpha-methyltyrosine (0.5 mM) for 40 hr, catecholamine levels were depressed to an extent similar to that observed with DNLCA. Incubation with [3H]tyrosine in the presence of TIQCAs revealed inhibition of tyrosine uptake and suggested a reduction in the rate of catecholamine synthesis. These results are consistent with previous data on the inhibition of tyrosine 3-monooxygenase by DNLCA in vitro.
M P Galloway; W J Burke; C J Coscia
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Biochemical pharmacology     Volume:  31     ISSN:  0006-2952     ISO Abbreviation:  Biochem. Pharmacol.     Publication Date:  1982 Oct 
Date Detail:
Created Date:  1983-01-19     Completed Date:  1983-01-19     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0101032     Medline TA:  Biochem Pharmacol     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  3251-6     Citation Subset:  IM    
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MeSH Terms
Adrenal Medulla / metabolism*
Catecholamines / metabolism*
Dopamine / metabolism
Epinephrine / metabolism
Isoquinolines / pharmacology*
Norepinephrine / metabolism
Organ Culture Techniques
Proteins / metabolism
Tetrahydropapaveroline / analogs & derivatives,  pharmacology
Tyrosine / metabolism
Grant Support
Reg. No./Substance:
0/3',4'-deoxynorlaudanosolinecarboxylic acid; 0/Catecholamines; 0/Isoquinolines; 0/Proteins; 4747-99-3/Tetrahydropapaveroline; 51-41-2/Norepinephrine; 51-43-4/Epinephrine; 55520-40-6/Tyrosine; 65427-91-0/norlaudanosoline-1-carboxylic acid

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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