Document Detail


Tetanus toxin action: inhibition of neurotransmitter release linked to synaptobrevin proteolysis.
MedLine Citation:
PMID:  1361727     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Tetanus toxin is a potent neurotoxin that inhibits the release of neurotransmitters from presynaptic nerve endings. The mature toxin is composed of a heavy and a light chain that are linked via a disulfide bridge. After entry of tetanus toxin into the cytoplasm, the released light chain causes block of neurotransmitter release. Recent evidence suggests that the L-chain may act as a metalloendoprotease. Here we demonstrate that blockade of neurotransmission by tetanus toxin in isolated nerve terminals is associated with a selective proteolysis of synaptobrevin, an integral membrane protein of synaptic vesicles. No other proteins appear to be affected by tetanus toxin. In addition, recombinant light chain selectively cleaves synaptobrevin when incubated with purified synaptic vesicles. Our data suggest that cleavage of synaptobrevin is the molecular mechanism of tetanus toxin action.
Authors:
E Link; L Edelmann; J H Chou; T Binz; S Yamasaki; U Eisel; M Baumert; T C Südhof; H Niemann; R Jahn
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Biochemical and biophysical research communications     Volume:  189     ISSN:  0006-291X     ISO Abbreviation:  Biochem. Biophys. Res. Commun.     Publication Date:  1992 Dec 
Date Detail:
Created Date:  1993-01-28     Completed Date:  1993-01-28     Revised Date:  2005-11-17    
Medline Journal Info:
Nlm Unique ID:  0372516     Medline TA:  Biochem Biophys Res Commun     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1017-23     Citation Subset:  IM    
Affiliation:
Department of Pharmacology, Yale University Medical School, New Haven, CT 06510.
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MeSH Terms
Descriptor/Qualifier:
Animals
Calcium / pharmacology
Dose-Response Relationship, Drug
Egtazic Acid / pharmacology
Glutamates / metabolism*
Glutamic Acid
Kinetics
Macromolecular Substances
Membrane Proteins / isolation & purification,  metabolism*
Nerve Tissue Proteins / isolation & purification,  metabolism*
Neurotoxins / pharmacology*
Neurotransmitter Agents / metabolism*
R-SNARE Proteins
Synaptic Vesicles / drug effects,  metabolism*
Synaptosomes / drug effects,  metabolism*
Tetanus Toxin / pharmacology*
Chemical
Reg. No./Substance:
0/Glutamates; 0/Macromolecular Substances; 0/Membrane Proteins; 0/Nerve Tissue Proteins; 0/Neurotoxins; 0/Neurotransmitter Agents; 0/R-SNARE Proteins; 0/Tetanus Toxin; 56-86-0/Glutamic Acid; 67-42-5/Egtazic Acid; 7440-70-2/Calcium

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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