| Tetanus antitoxin binds to intracellular tetanus toxin in permeabilized chromaffin cells without restoring Ca2(+)-induced exocytosis. | |
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MedLine Citation:
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PMID: 2260105 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Tetanus toxin blocks Ca2(+)-evoked catecholamine release from permeabilized bovine adrenal chromaffin cells preloaded with gangliosides. Tetanus toxin preincubated with its specific antibodies F(ab')2 is without any effect on exocytosis. Specific antitetanus F(ab')2 presented to chromaffin cells which are pretreated with tetanus toxin and permeabilized by digitonin cannot restore exocytosis. Under the same conditions, however, 125I-labeled F(ab')2 accumulates in chromaffin cells. The accumulation depends on the presence and concentration of tetanus toxin and can be prevented by an excess of unlabeled F(ab')2. Once tetanus toxin has initiated block of exocytosis, it cannot be neutralized by binding to its specific antibody. |
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Authors:
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P Marxen; G Ahnert-Hilger; H H Wellhöner; H Bigalke |
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Publication Detail:
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Type: In Vitro; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Toxicon : official journal of the International Society on Toxinology Volume: 28 ISSN: 0041-0101 ISO Abbreviation: Toxicon Publication Date: 1990 |
Date Detail:
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Created Date: 1991-01-25 Completed Date: 1991-01-25 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 1307333 Medline TA: Toxicon Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 1077-82 Citation Subset: IM |
Affiliation:
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Medical School of hannover, Dept of Pharmacology and Toxicology, F.R.G. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Calcium / physiology* Cattle Chromaffin Granules / drug effects, metabolism* Exocytosis / drug effects* Gangliosides / physiology Immunoglobulin Fab Fragments / metabolism Norepinephrine / secretion Tetanus Antitoxin / metabolism* Tetanus Toxin / metabolism, pharmacology* |
| Chemical | |
Reg. No./Substance:
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0/Gangliosides; 0/Immunoglobulin Fab Fragments; 0/Tetanus Antitoxin; 0/Tetanus Toxin; 51-41-2/Norepinephrine; 7440-70-2/Calcium |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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