Document Detail


Testosterone treatment results in quiescent satellite cells being activated and recruited into cell cycle in rat levator ani muscle.
MedLine Citation:
PMID:  7750644     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
At puberty, the male levator ani (LA) muscle exhibits muscle fiber hypertrophy. This fiber enlargement can be provoked in the female LA muscle by testosterone treatment. In both cases the hypertrophic process is accompanied by an increase in the number of satellite cells and myonuclei. The present ultrastructural autoradiographic study was undertaken in order to investigate (1) whether satellite cells when stimulated by testosterone can undergo DNA synthesis by incorporating [3H]thymidine ([3H]Tdr); (2) whether the whole satellite cell population is committed in the cell cycle; and (3) whether the new myonuclei originate from fusion of the satellite cells with the preexistant myofibers. Thirty-day-old female rats treated with a single testosterone injection received a single injection of [3H]Tdr at 24, 28, 32, to 34 hr after testosterone treatment. LA muscles were removed 2 hr after [3H]Tdr injection. This first series of experiments allowed us to determine that onset of DNA synthesis in satellite cells occurs within the 34th and the 36th hour after testosterone treatment. To obtain a more precise timing, 30-day-old female rats treated with a single testosterone injection received multiple injections every 30 min either from Hour 32 to 33.5 or from Hour 34 to 35.5 LA muscles were removed 30 min after the last [3H]Tdr injection. This showed that the onset of satellite cell replication occurred between the 32nd and the 34th hour after testosterone treatment. However, only 30% of the satellite cell population was affected by this proliferative process regardless of the experimental protocol used. To confirm that the increase in myonuclei number results from incorporation of satellite cells into mature myofibers, 30-day-old female rats treated with a single injection of testosterone received a single injection of [3H]Tdr on the 60th hour after testosterone treatment. LA muscles were removed at 63, 84, to 108 hr after [3H]Tdr injection. We conclude that testosterone induces satellite cell proliferation at around the 33rd hour and that the increased number of myonuclei reported in our previous study is due to fusion of labeled satellite cells with myofibers.
Authors:
Y Joubert; C Tobin
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Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Developmental biology     Volume:  169     ISSN:  0012-1606     ISO Abbreviation:  Dev. Biol.     Publication Date:  1995 May 
Date Detail:
Created Date:  1995-06-16     Completed Date:  1995-06-16     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0372762     Medline TA:  Dev Biol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  286-94     Citation Subset:  IM    
Affiliation:
Laboratoire de Neurobiologie des signaux intercellulaires, URA 1488, Paris.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Cycle / drug effects*
Female
Male
Muscles / cytology*,  drug effects*,  ultrastructure
Rats
Rats, Wistar
Sex Characteristics
Stem Cells / drug effects*
Testosterone / pharmacology*
Chemical
Reg. No./Substance:
58-22-0/Testosterone

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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