| Testosterone-induced muscle hypertrophy is associated with an increase in satellite cell number in healthy, young men. | |
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MedLine Citation:
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PMID: 12670837 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Testosterone (T) supplementation in men induces muscle fiber hypertrophy. We hypothesized that T-induced increase in muscle fiber size is associated with a dose-dependent increase in satellite cell number. We quantitated satellite cell and myonuclear number by using direct counting and spatial orientation methods in biopsies of vastus lateralis obtained at baseline and after 20 wk of treatment with a gonadotropin-releasing hormone agonist and a 125-, 300-, or 600-mg weekly dose of T enanthate. T administration was associated with a significant increase in myonuclear number in men receiving 300- and 600-mg doses. The posttreatment percent satellite cell number, obtained by direct counting, differed significantly among the three groups (ANCOVA P < 0.000001); the mean posttreatment values (5.0 and 15.0%) in men treated with 300- and 600-mg doses were greater than baseline (2.5 and 2.5%, respectively, P < 0.05 vs. baseline). The absolute satellite cell number measured by spatial orientation at 20 wk (1.5 and 4.0/mm) was significantly greater than baseline (0.3 and 0.6/mm) in men receiving the 300- and 600-mg doses (P < 0.05). The change in percent satellite cell number correlated with changes in total (r = 0.548) and free T concentrations (r = 0.468). Satellite cell and mitochondrial areas were significantly higher and the nuclear-to-cytoplasmic ratio lower after treatment with 300- and 600-mg doses. We conclude that T-induced muscle fiber hypertrophy is associated with an increase in satellite cell number, a proportionate increase in myonuclear number, and changes in satellite cell ultrastructure. |
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Authors:
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Indrani Sinha-Hikim; Stephen M Roth; Martin I Lee; Shalender Bhasin |
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Publication Detail:
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Type: Clinical Trial; Journal Article; Randomized Controlled Trial; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. Date: 2003-04-01 |
Journal Detail:
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Title: American journal of physiology. Endocrinology and metabolism Volume: 285 ISSN: 0193-1849 ISO Abbreviation: Am. J. Physiol. Endocrinol. Metab. Publication Date: 2003 Jul |
Date Detail:
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Created Date: 2003-06-06 Completed Date: 2003-07-18 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 100901226 Medline TA: Am J Physiol Endocrinol Metab Country: United States |
Other Details:
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Languages: eng Pagination: E197-205 Citation Subset: IM |
Affiliation:
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Division of Endocrinology, Metabolism, and Molecular Medicine, Charles R. Drew University of Medicine and Science, Los Angeles, California 90059, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adult Cell Count Cell Nucleus / drug effects Cell Size / drug effects Double-Blind Method Gonadotropin-Releasing Hormone / pharmacology Humans Male Microscopy, Electron Muscle Fibers, Fast-Twitch / physiology, ultrastructure Muscle, Skeletal / cytology*, drug effects, growth & development* Satellite Cells, Skeletal Muscle / drug effects* Testosterone / analogs & derivatives*, blood, pharmacology, physiology* Tissue Fixation |
| Grant Support | |
ID/Acronym/Agency:
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1R01 AG-14369-01/AG/NIA NIH HHS; 1R01 DK-59627-01/DK/NIDDK NIH HHS; 2R01 DK-49296-02A/DK/NIDDK NIH HHS; 5F32 AG-05893-02/AG/NIA NIH HHS; G12 RR-03026/RR/NCRR NIH HHS; M01 RR-00425/RR/NCRR NIH HHS; P20 RR-11145/RR/NCRR NIH HHS; U01 DK-54047/DK/NIDDK NIH HHS; U54 RR-14616/RR/NCRR NIH HHS |
| Chemical | |
Reg. No./Substance:
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315-37-7/testosterone enanthate; 33515-09-2/Gonadotropin-Releasing Hormone; 58-22-0/Testosterone |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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