| TESTOSTERONE EXERTS ANTI-APOPTOTIC EFFECTS AGAINST H2O2 IN C2C12 SKELETAL MUSCLE CELLS THROUGH THE APOPTOTIC INTRINSIC PATHWAY. | |
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MedLine Citation:
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PMID: 22219300 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Experimental data indicate that apoptosis is activated in the aged skeletal muscle, contributing to sarcopenia. We have previously demonstrated that testosterone protects against H2O2-induced apoptosis in C2C12 muscle cells. Here we identified molecular events involved in the anti-apoptotic effect of testosterone. At short times of exposure to H2O2 cells exhibit a defense response but at longer treatment times cells undergo into apoptosis. Incubation with testosterone prior to H2O2, induces Bad inactivation, inhibition of PARP cleavage, a decrease in Bax levels and impedes the loss of mitochondrial membrane potential, suggesting that the hormone participates in the regulation of the apoptotic intrinsic pathway. Simultaneous treatment with testosterone, H2O2 and the AR antagonist, flutamide, reduce the effects of the hormone, pointing to a possible participation of the AR in the anti-apoptotic effect. The data presented allow us to begin to elucidate the mechanism by which the hormone prevents apoptosis in skeletal muscle. |
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Authors:
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Lucía Pronsato; Ricardo Boland; Lorena M Milanesi |
Publication Detail:
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Type: JOURNAL ARTICLE Date: 2012-1-4 |
Journal Detail:
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Title: The Journal of endocrinology Volume: - ISSN: 1479-6805 ISO Abbreviation: - Publication Date: 2012 Jan |
Date Detail:
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Created Date: 2012-1-5 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0375363 Medline TA: J Endocrinol Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
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L Pronsato, Departamento de Biología, Bioquímica y Farmacia, Universidad Nacional del Sur, Bahía Blanca, Argentina. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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