| Testosterone decreases ornithine decarboxylase messenger RNA levels in primary cultures of rat Sertoli cells. | |
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MedLine Citation:
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PMID: 2293029 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We have previously reported that testosterone decreased ornithine decarboxylase (ODC) activity in primary cultures of rat Sertoli cells. In this report we examined the mechanism of this reduction. In cells pretreated with testosterone (5 x 10(-7) M) for 48 h before the start of the experiment ODC activity was decreased, on the average, 43% at all time points examined. ODC mRNA levels were also decreased an overall 33%. The testosterone-mediated decrease in ODC activity was first seen 8 h after the addition of testosterone to the cells. Testosterone had no significant affect on the levels of actin or transferrin mRNA. The effect of testosterone was androgen specific. Neither ODC activity nor mRNA was affected by the nonandrogenic steroids progesterone or cortisol. These results suggest that testosterone decreases ODC mRNA in Sertoli cells either through an inhibition of transcription or through a decrease in message stability. Testosterone does not appear to affect ODC mRNA translation, since the percent decreases in ODC activity and mRNA in response to testosterone were essentially equivalent. Regulation of Sertoli cell ODC expression by testosterone may reflect one mechanism by which Sertoli cell function is integrated with surrounding cell types. The Sertoli cell, unlike any other cell, secretes putrescine, the product of ODC catalysis of ornithine. We suggest that the modulation of ODC by testosterone and, hence, the amount of putrescine secreted by the Sertoli cell may be significant in the process of spermatogenesis. |
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Authors:
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K X Weiner; B T Pentecost; J A Dias |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Molecular endocrinology (Baltimore, Md.) Volume: 4 ISSN: 0888-8809 ISO Abbreviation: Mol. Endocrinol. Publication Date: 1990 Aug |
Date Detail:
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Created Date: 1991-04-09 Completed Date: 1991-04-09 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 8801431 Medline TA: Mol Endocrinol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 1249-56 Citation Subset: IM |
Affiliation:
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Department of Biochemistry, Albany Medical College, New York 12208. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cells, Cultured Dihydrotestosterone / pharmacology Estradiol / pharmacology Hydrocortisone / pharmacology Male Ornithine Decarboxylase / genetics*, metabolism Progesterone / pharmacology Putrescine / metabolism RNA, Messenger / metabolism* Rats Rats, Inbred Strains Sertoli Cells / drug effects, enzymology* Testosterone / pharmacology* Transcription, Genetic / drug effects |
| Grant Support | |
ID/Acronym/Agency:
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2S07-RR-05649-22/RR/NCRR NIH HHS; HD-00682/HD/NICHD NIH HHS; S07-RR-05394-25/RR/NCRR NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/RNA, Messenger; 110-60-1/Putrescine; 50-23-7/Hydrocortisone; 50-28-2/Estradiol; 521-18-6/Dihydrotestosterone; 57-83-0/Progesterone; 58-22-0/Testosterone; EC 4.1.1.17/Ornithine Decarboxylase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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