Document Detail

Testis-specific TTF-D binds to single-stranded DNA in the c-mos and Odf1 promoters and activates Odf1.
MedLine Citation:
PMID:  10206985     Owner:  NLM     Status:  MEDLINE    
We recently identified testis-specific nuclear factor binding sites in the testis-specific promoters of the c-mos gene and the Odf1 gene, which are 80% identical. Here we characterize a testis-specific nuclear factor, TTF-D, which is able to complex with both binding sites and stimulates Odf1 promoter activity. TTF-D is detectable in mouse testis as early as day 11 postpartum and contains three peptides of 22, 25, and 35 kDa in size. Surprisingly, TTF-D binds specifically to its cognate double-stranded DNA binding site as well as to its single-stranded DNA binding site. Both double-stranded and single-stranded binding site oligonucleotide DNA can specifically repress Odf1 promoter activity. Our results suggest that TTF-D is involved in positive transcription regulation of a pre-meiotic and a post-meiotic gene in the testis.
J H Oosterhuis; F A van der Hoorn
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Journal of biological chemistry     Volume:  274     ISSN:  0021-9258     ISO Abbreviation:  J. Biol. Chem.     Publication Date:  1999 Apr 
Date Detail:
Created Date:  1999-05-20     Completed Date:  1999-05-20     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  2985121R     Medline TA:  J Biol Chem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  11708-12     Citation Subset:  IM    
Department of Biochemistry & Molecular Biology, University of Calgary, 3330 Hospital Drive NW, Calgary, Alberta T2N 4N1, Canada.
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MeSH Terms
Base Sequence
DNA Primers
DNA, Single-Stranded / metabolism*
DNA-Binding Proteins / genetics,  metabolism
Gene Expression Regulation, Developmental
Heat-Shock Proteins*
Nuclear Proteins / genetics,  metabolism*
Promoter Regions, Genetic*
Protein Binding
Proteins / genetics*
Proto-Oncogene Proteins c-mos / genetics*
Testis / metabolism*
Transcription Factors / genetics,  metabolism
Reg. No./Substance:
0/DNA Primers; 0/DNA, Single-Stranded; 0/DNA-Binding Proteins; 0/Heat-Shock Proteins; 0/Nuclear Proteins; 0/Odf1 protein, mouse; 0/Odf1 protein, rat; 0/Proteins; 0/Transcription Factors; EC Proteins c-mos

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