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Testing prey DNA fingerprinting on Amblyseius largoensis (Acari: Phytoseiidae) predation of Raoiella indica (Acari: Tenuipalpidae).
MedLine Citation:
PMID:  22476445     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Molecular detection of predation by identifying prey markers in the digestive tract of predators has developed into a powerful tool to assess predator-prey systems in which diet identification is too time consuming or impossible. Here we explore its utility for detecting predation of the pest mite Raoiella indica Hirst by the predatory mite Amblyseius largoensis Muma, taking advantage of the color the predator acquires after eating this mite to cross-reference our results. For this, a ~410 bp segment of the cytochrome c oxidase subunit I (COI) mitochondrial gene marker specific for the subfamily Tetranychoidea was used. Amblyseius largoensis that had recently eaten were collected from greenhouse colonies containing both mites, and isolated from any other food source. Predator mites were taken for fingerprinting at 24, 48, 72 and 96 h of starving after collection, and the same process was repeated a second time, offering pollen as an alternative food source to see whether detection changed. Lastly, a sampling trial was conducted in the greenhouse, in which mites were collected regardless of their color and frozen immediately for fingerprinting. Raoiella indica DNA was detected for 48 h on starving predators, and for 96 h on those who had eaten pollen. The segment was detected in 26 % of the samples collected on the trial. This technique needs refinement specific for this system, but the results obtained here confirm that it could turn into a very useful tool for assessing aspects of this predator-prey system.
Authors:
Carlos Rivera-Rivera; Alberto Galindo-Cardona; Jose Carlos Verle Rodrigues
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-4-5
Journal Detail:
Title:  Experimental & applied acarology     Volume:  -     ISSN:  1572-9702     ISO Abbreviation:  -     Publication Date:  2012 Apr 
Date Detail:
Created Date:  2012-4-5     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8507436     Medline TA:  Exp Appl Acarol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
Agricultural Experimental Station Río Piedras, Crops and AgroEnvironmental Sciences Department, University of Puerto Rico, 1193, Calle Guayacan, San Juan, PR, 00926, USA.
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