| Testing neocortical slice viability in non-perfused no-magnesium artificial cerebrospinal fluid solutions. | |
| | |
MedLine Citation:
|
PMID: 22178217 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
|
The acute in vitro brain slice model is a widely used neurophysiological research tool. When applying this method, most researchers continuously perfuse slices with carbogenated artificial cerebrospinal fluid (ACSF) to maintain pH balance and tissue oxygen delivery. Common wisdom suggests that static recordings are incompatible with submerged bath methodology because of deficiency in tissue oxygen supply. However, to our knowledge this has not been tested. In this study, we wanted to determine whether neocortical mouse slice viability could be maintained in the medium term (up to 2h) in a shallow, submerged recording bath under non-perfused, static conditions. Seizure-like events (SLEs) were generated in the slices utilizing no-magnesium ACSF and recorded for 2h under three conditions: (1) perfused ACSF condition (n=8), where slices were perfused continuously with carbogenated no-magnesium ACSF; (2) static ACSF condition (n=12), where slices were recorded in pre-carbogenated, but non-perfused (static) no-magnesium ACSF; and (3) static HEPES ACSF condition (n=12), where slices were recorded in non-perfused (static) no-magnesium ACSF with no pre-carbogenation but buffered with HEPES. SLE activity was stable for 2h across all three conditions. There was no statistically significant difference in SLE frequency, amplitude or length between static and perfused conditions. SLE frequency and amplitude were generally lower in the static HEPES buffer condition. The data indicate that robust and stable neocortical SLE activity can be generated for at least 2h in a submersion bath without ACSF perfusion if pH is adequately controlled. |
| | |
Authors:
|
Logan J Voss; Shwetha A George; James W Sleigh |
Related Documents
:
|
20488877 - Ferrochelatase is a conserved downstream target of the blue light-sensing white collar ... 20031917 - A rice phytochrome a in arabidopsis: the role of the n-terminus under red and far-red l... 8458347 - Transduction of the light signal during complementary chromatic adaptation in the cyano... 7498777 - Environmental programming of heritable epigenetic changes in paramutant r-gene expressi... 11011897 - Optimization characteristics of walking with and without a load on the trunk of the body. 12843397 - Altered patterns of sleep and behavioral adaptability in npas2-deficient mice. |
Publication Detail:
|
Type: JOURNAL ARTICLE Date: 2011-12-8 |
Journal Detail:
|
Title: Journal of neuroscience methods Volume: - ISSN: 1872-678X ISO Abbreviation: - Publication Date: 2011 Dec |
Date Detail:
|
Created Date: 2011-12-19 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 7905558 Medline TA: J Neurosci Methods Country: - |
Other Details:
|
Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
|
Copyright © 2011. Published by Elsevier B.V. |
Affiliation:
|
Anesthesia Department, Waikato District Health Board, Hamilton, New Zealand. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Role of Differentiation of Liver Sinusoidal Endothelial Cells in Progression and Regression of Hepat...
Next Document: Is zinc-?2-glycoprotein a cardiovascular protective factor for patients undergoing hemodialysis?