| Testing biomaterials by the in-situ evaluation of cell response. | |
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MedLine Citation:
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PMID: 12202189 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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In this work, we describe a technique for the in-situ observation of cells adhered on opaque biomaterial surfaces. The visualisation of the morphology of cells adhered onto a surface allows to derive nuclear apoptotic signs or even the existence of organisation between groups of these cells. The technique is based on the use of an auto-immune reaction combined with a fluorescent agent that allows a direct inspection of the cell behaviour. The versatility of the technique is demonstrated by presenting several examples with different cultured cells (human chondrosarcome cells (CSRCs) and pluripotential mesenchymal stem cells (MSCs) from bone marrow) seeded over two different Ti-based surfaces (TiO(2) and TiN, respectively). These in-vitro observations are compared with the behaviour of the same cells on bare TiAlV alloy. From our results it is concluded that both TiO(2) and TiN surfaces show enhanced biological responses. |
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Authors:
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M Manso; S Ogueta; J Pérez-Rigueiro; J P García; J M Martínez-Duart |
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Publication Detail:
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Type: Comparative Study; Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Biomolecular engineering Volume: 19 ISSN: 1389-0344 ISO Abbreviation: Biomol. Eng. Publication Date: 2002 Aug |
Date Detail:
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Created Date: 2002-08-30 Completed Date: 2003-03-07 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 100928062 Medline TA: Biomol Eng Country: Netherlands |
Other Details:
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Languages: eng Pagination: 239-42 Citation Subset: IM |
Affiliation:
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Dpto. Física Aplicada C-XII, Universidad Autónoma de Madrid, 28049 Madrid, Spain. miguel.manso@uam.es |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Cell Adhesion
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immunology Cell Division Cells, Cultured Chondrosarcoma / immunology*, pathology* Coated Materials, Biocompatible Cytoskeleton Fluorescence Polarization Immunoassay / methods Hematopoietic Stem Cells / cytology*, immunology* Humans Materials Testing / methods* Mesoderm / immunology Titanium* |
| Chemical | |
Reg. No./Substance:
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0/Coated Materials, Biocompatible; 11116-16-8/titanium nitride; 12743-70-3/titanium alloy (TiAl6V4); 13463-67-7/titanium dioxide; 7440-32-6/Titanium |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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