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Testing photobiomodulatory effects of laser irradiation on wound healing: development of an improved model for dressing wounds in mice.
MedLine Citation:
PMID:  20666635     Owner:  NLM     Status:  In-Process    
Abstract/OtherAbstract:
OBJECTIVE: To develop a suitable method for dressing skin wounds in BKS.Cg-m(+)/(+)Lepr(db) mice for subsequent use in laser irradiation of wounds. The healing of nonirradiated wounds (controls) was examined histologically to provide essential reference data.
BACKGROUND DATA: Dressing excisional skin wounds in mice has many advantages. However, previous studies using dressings such as Tegaderm W or OpSite, with or without adhesives, have shown that this is not easily achieved.
MATERIALS AND METHODS: In a pilot study, a full-thickness wound was made on the left flank in six diabetic and six nondiabetic mice, and five different methods were tried for dressing the wounds with Tegaderm HP to develop an optimized procedure. The optimized procedure was used in subsequent studies, with a total of 23 diabetic and 13 nondiabetic mice being controls for laser-irradiated mice. Measurements of healing outcomes from histologic sections of controls were statistically analyzed.
RESULTS: The optimized procedure used Tegaderm HP with Cavilon and Fixomull Stretch strips for the first dressing, and with Mastisol for subsequent dressings. Wound closure by contraction was retarded in a large proportion of diabetic mice (approximately 80%) and a small proportion of nondiabetic mice. These wounds, described as "splinted," healed mainly by epithelial regeneration and granulation tissue formation.
CONCLUSION: A simple, easy-to-perform procedure was developed for dressing wounds in diabetic and nondiabetic mice. It was found to cause splinting with wound healing mimicking that in human patients. This model is suitable for examining the effects of different therapies on wound healing, including lasers.
Authors:
Tzu-Yun Chung; Philip V Peplow; G David Baxter
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Publication Detail:
Type:  Journal Article     Date:  2010-07-28
Journal Detail:
Title:  Photomedicine and laser surgery     Volume:  28     ISSN:  1557-8550     ISO Abbreviation:  Photomed Laser Surg     Publication Date:  2010 Oct 
Date Detail:
Created Date:  2010-10-21     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101222340     Medline TA:  Photomed Laser Surg     Country:  United States    
Other Details:
Languages:  eng     Pagination:  589-96     Citation Subset:  IM    
Affiliation:
Department of Anatomy & Structural Biology, School of Physiotherapy, University of Otago, Dunedin, New Zealand.
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