| Testicular isoform of angiotensin I-converting enzyme (ACE, CD143) on the surface of human spermatozoa: revelation and quantification using monoclonal antibodies. | |
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MedLine Citation:
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PMID: 16364013 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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PROBLEM: The elucidation of the role of angiotensin-converting enzyme (ACE, CD143) in the male fertility has been hampered by the absence of highly specific antibodies to the native testicular isoform (tACE). The quantification of tACE expression on human-ejaculated spermatozoa was performed using a novel panel of monoclonal antibodies (mAbs). METHOD OF STUDY: The expression of tACE on the surface of live and fixed human spermatozoa was analyzed by flow cytometry and immunocytochemistry using new mAbs to human tACE. RESULTS: Monoclonal antibodies 1E10 and 4E3 similarly revealed tACE on the surface of live and fixed spermatozoa. The high percentage of tACE-positive spermatozoa (median 81%) was revealed in the swim-up fraction of sperm. Antibody-induced tACE shedding occurs preferentially from live sperm with defective function and/or morphology. Testicular ACE is located on the plasma membrane of the post-acrosomal region, the neck and midpiece of normal spermatozoa, but showed a variable distribution on the defective cells. CONCLUSIONS: The new mAbs recognizing the C-terminal domain of human ACE are useful tools for quantification of tACE expression on human live and fixed spermatozoa and further adequate analysis of the tACE role in reproduction. |
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Authors:
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Marina A Nikolaeva; Irina V Balyasnikova; Marina A Alexinskaya; Roman Metzger; Folker E Franke; Ronald F Albrecht; Vladimir I Kulakov; Gennady T Sukhikh; Sergei M Danilov |
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Publication Detail:
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Type: Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: American journal of reproductive immunology (New York, N.Y. : 1989) Volume: 55 ISSN: 1046-7408 ISO Abbreviation: Am. J. Reprod. Immunol. Publication Date: 2006 Jan |
Date Detail:
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Created Date: 2005-12-20 Completed Date: 2006-03-31 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 8912860 Medline TA: Am J Reprod Immunol Country: Denmark |
Other Details:
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Languages: eng Pagination: 54-68 Citation Subset: IM |
Affiliation:
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Research Center for Obstetrics, Gynecology and Perinatology, Moscow, Russia. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Antibodies, Monoclonal* CHO Cells Cell Membrane / metabolism Cells, Cultured Cricetinae Ejaculation Flow Cytometry Humans Immunohistochemistry Male Peptidyl-Dipeptidase A / immunology, metabolism* Sperm Motility Spermatozoa / metabolism* |
| Chemical | |
Reg. No./Substance:
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0/Antibodies, Monoclonal; EC 3.4.15.1/ACE protein, human; EC 3.4.15.1/Peptidyl-Dipeptidase A |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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