| Testicular apoptosis after dietary zinc deficiency: Ultrastructural and TUNEL studies. | |
| | |
MedLine Citation:
|
PMID: 21644846 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
|
The present study was conducted in Wistar rats to determine whether prepubertal dietary zinc deficiency causes apoptotic changes in testes. Prepubertal male Wistar rats (40-50 gm) were divided into 3 groups: zinc control (ZC), pairfed (PF), and zinc deficient (ZD). Control and pairfed groups were given a 100 ppm zinc diet while the deficient groups received 1 ppm zinc diet for 2 and 4 weeks (w), respectively. Ultrastructural studies revealed several apoptotic features such as wavy basement membrane, displaced nuclei, chromatin condensation, plasma membrane blebbing, nuclear membrane dissolution, loss of inter-Sertoli cell junctional complexes, and intercellular bridges and deformed mitochondria. A variable spectrum of sperm defects had also been visualized e.g., acrosomal deformities such as decapitation and a ring of condensed chromatin around the nuclear periphery, deformed sperm heads with a condensed nucleus, tail-elements with superfluous cytoplasm, and damage to the mitochondrial sheath and aggregation of spermatozoa within the membrane. This was further supported by TUNEL studies. Apoptotic index, epididymal sperm concentration, motility, and fertility index also revealed a significant (P < 0.05) decrease in zinc deficient groups (2 and 4 w) when compared with their respective control and pairfed groups. All the above findings are indicative that changes observed in the testes after dietary zinc deficiency are due to the onset of apoptosis. Increased apoptotic degeneration in testes may cause irreversible changes in the germ cells associated with decreased epididymal sperm concentration, motility, and fertility index which contributes to the low efficiency of spermatogenesis thereby indicating a possible role of zinc in fertility. |
| | |
Authors:
|
Deepa Kumari; Neena Nair; Ranveer Singh Bedwal |
Related Documents
:
|
16174366 - The manufacture of miniature cheddar-type cheeses from milks with different fat globule... 19307626 - Effects of milking interval and cisternal udder evaluation in tunisian maghrebi dairy d... 20416656 - Effect of suckling management on productive performance, carcass traits and meat qualit... 15453506 - Increasing amounts of conjugated linoleic acid progressively reduces milk fat synthesis... 22612956 - Rumination and its relationship to feeding and lying behavior in holstein dairy cows. 16750506 - Miniaturization of asymmetrical flow field-flow fractionation and application to studie... |
Publication Detail:
|
Type: JOURNAL ARTICLE Date: 2011-6-7 |
Journal Detail:
|
Title: Systems biology in reproductive medicine Volume: - ISSN: 1939-6376 ISO Abbreviation: - Publication Date: 2011 Jun |
Date Detail:
|
Created Date: 2011-6-7 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 101464963 Medline TA: Syst Biol Reprod Med Country: - |
Other Details:
|
Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
|
Cell Biology Laboratory, Department of Zoology, University of Rajasthan , Jaipur , India. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: A pilot study of the impact of genotype on nifedipine pharmacokinetics when used as a tocolytic.
Next Document: Evaluation of vitamin D levels among HIV-infected patients in New York City.