Document Detail

Terpenoids induce cell cycle arrest and apoptosis from the stems of Celastrus kusanoi associated with reactive oxygen species.
MedLine Citation:
PMID:  20178391     Owner:  NLM     Status:  MEDLINE    
Bioguided fractionation of the CHCl(3) extracts obtained from Celastrus kusanoi stems led to isolation of two new terpenoids, 3beta-hydroxy-11,14-oxo-abieta-8,12-diene (1) and 3beta-trans-(3,4-dihydroxycinnamoyloxy)-11alpha-methoxy-12-ursene (2), and four known compounds characterized by spectroscopic methods. Compounds 1 and 2 and known triterpenoid erythrodiol (3) exhibited cytotoxic activity against bladder cancer cells (NTUB1) with IC(50) values of 58.2 +/- 2.3, 160.1 +/- 60.9, and 18.3 +/- 0.5 microM, respectively. Exposure of NTUB1 to 3 (5 and 10 microM) for 24 h significantly increased the level of production of reactive oxygen species (ROS). Flow cytometric analysis showed that treatment of NTUB1 with 3 led to the cell cycle arrest at G0/G1 accompanied by an increase in the extent of apoptotic cell death after 24 h. These data suggest that the presentation of G1 phase arrest and apoptosis in 3-treated NTUB1 for 24 h was mediated through an increased amount of ROS in cells exposed to 3.
Hui-Ling Chen; Kai-Wei Lin; A-Mei Huang; Huang-Yao Tu; Bai-Luh Wei; Tzyh-Chyuan Hour; Ming-Hong Yen; Yeong-Shiou Pu; Chun-Nan Lin
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of agricultural and food chemistry     Volume:  58     ISSN:  1520-5118     ISO Abbreviation:  J. Agric. Food Chem.     Publication Date:  2010 Mar 
Date Detail:
Created Date:  2010-03-17     Completed Date:  2010-07-15     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0374755     Medline TA:  J Agric Food Chem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  3808-12     Citation Subset:  IM    
Faculty of Pharmacy, College of Pharmacy, Kaohsiung Medical University, Kaohsiung, Taiwan.
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MeSH Terms
Apoptosis / drug effects*
Celastrus / chemistry*
Cell Cycle / drug effects*
Cell Line, Tumor
Plant Extracts / pharmacology*
Plant Stems / chemistry
Reactive Oxygen Species / metabolism*
Terpenes / pharmacology*
Urinary Bladder Neoplasms / drug therapy,  metabolism,  physiopathology
Reg. No./Substance:
0/Plant Extracts; 0/Reactive Oxygen Species; 0/Terpenes

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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