| Terpenoids induce cell cycle arrest and apoptosis from the stems of Celastrus kusanoi associated with reactive oxygen species. | |
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MedLine Citation:
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PMID: 20178391 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Bioguided fractionation of the CHCl(3) extracts obtained from Celastrus kusanoi stems led to isolation of two new terpenoids, 3beta-hydroxy-11,14-oxo-abieta-8,12-diene (1) and 3beta-trans-(3,4-dihydroxycinnamoyloxy)-11alpha-methoxy-12-ursene (2), and four known compounds characterized by spectroscopic methods. Compounds 1 and 2 and known triterpenoid erythrodiol (3) exhibited cytotoxic activity against bladder cancer cells (NTUB1) with IC(50) values of 58.2 +/- 2.3, 160.1 +/- 60.9, and 18.3 +/- 0.5 microM, respectively. Exposure of NTUB1 to 3 (5 and 10 microM) for 24 h significantly increased the level of production of reactive oxygen species (ROS). Flow cytometric analysis showed that treatment of NTUB1 with 3 led to the cell cycle arrest at G0/G1 accompanied by an increase in the extent of apoptotic cell death after 24 h. These data suggest that the presentation of G1 phase arrest and apoptosis in 3-treated NTUB1 for 24 h was mediated through an increased amount of ROS in cells exposed to 3. |
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Authors:
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Hui-Ling Chen; Kai-Wei Lin; A-Mei Huang; Huang-Yao Tu; Bai-Luh Wei; Tzyh-Chyuan Hour; Ming-Hong Yen; Yeong-Shiou Pu; Chun-Nan Lin |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of agricultural and food chemistry Volume: 58 ISSN: 1520-5118 ISO Abbreviation: J. Agric. Food Chem. Publication Date: 2010 Mar |
Date Detail:
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Created Date: 2010-03-17 Completed Date: 2010-07-15 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0374755 Medline TA: J Agric Food Chem Country: United States |
Other Details:
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Languages: eng Pagination: 3808-12 Citation Subset: IM |
Affiliation:
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Faculty of Pharmacy, College of Pharmacy, Kaohsiung Medical University, Kaohsiung, Taiwan. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Apoptosis
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drug effects* Celastrus / chemistry* Cell Cycle / drug effects* Cell Line, Tumor Humans Plant Extracts / pharmacology* Plant Stems / chemistry Reactive Oxygen Species / metabolism* Terpenes / pharmacology* Urinary Bladder Neoplasms / drug therapy, metabolism, physiopathology |
| Chemical | |
Reg. No./Substance:
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0/Plant Extracts; 0/Reactive Oxygen Species; 0/Terpenes |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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