Document Detail


Terminal long tandem repeats in chromosomes form Chironomus pallidivittatus.
MedLine Citation:
PMID:  8668143     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We provide evidence that a chromosome end in the dipteran Chironomus pallidivittatus contains 340-bp tandem repeats reaching the extreme terminus of the chromosome. After adding synthetic oligonucleotide tails to DNA extracted from the microdissected right end of the fourth chromosome, we could demonstrate that the blocks of repeats were tailed at only one end, the chromosome terminus, the interior of the arrays being unavailable for tailing. Using PCR, we furthermore showed that the added tails were connected to 340-bp repeat DNA directly, i.e., without intervening DNA of any other kind. The tailed repeats belong to a subfamily previously known to be the most peripheral one of the different types of 340-bp units. Using plasmid controls, we could also make certain that we did not amplify rare or nonrepresentative DNA termini.
Authors:
C C Löpez; L Nielsen; J E Edström
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Molecular and cellular biology     Volume:  16     ISSN:  0270-7306     ISO Abbreviation:  Mol. Cell. Biol.     Publication Date:  1996 Jul 
Date Detail:
Created Date:  1996-08-08     Completed Date:  1996-08-08     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  8109087     Medline TA:  Mol Cell Biol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  3285-90     Citation Subset:  IM    
Affiliation:
Department of Genetics, Lund University, Sweden.
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MeSH Terms
Descriptor/Qualifier:
Animals
Base Composition
Base Sequence
Chironomidae / genetics*
Chromosomes / chemistry*
Cloning, Molecular
Consensus Sequence
DNA / chemistry*
DNA Primers
Molecular Sequence Data
Plasmids
Polydeoxyribonucleotides
Polymerase Chain Reaction
Repetitive Sequences, Nucleic Acid*
Chemical
Reg. No./Substance:
0/DNA Primers; 0/Polydeoxyribonucleotides; 9007-49-2/DNA
Comments/Corrections

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