Document Detail

Teratogenic effects of azaspiracid-1 identified by microinjection of Japanese medaka (Oryzias latipes) embryos.
MedLine Citation:
PMID:  15904683     Owner:  NLM     Status:  MEDLINE    
Azaspiracid-1 (AZA-1) is a newly identified phycotoxin that accumulates in commercially important bivalve molluscs harvested in several European countries and causes severe human intoxications. Molluscan shellfish are known vectors for accumulation and subsequent transfer of phycotoxins such as brevetoxin and domoic acid through various trophic levels within food webs. Finfish can also accumulate phycotoxins, both directly from toxic algae or from consumption of contaminated shellfish and smaller intoxicated fish. To evaluate the teratogenic potential of AZA-1 and its relevancy to toxin accumulation in finfish, we have utilized a microinjection technique to mimic the maternal-egg toxin transfer of an AZA-1 reference standard and a shellfish extract containing azaspiracids in an embryonic Japanese medaka (Oryzias latipes) fish model. Microinjection of purified AZA-1 caused dose-dependent effects on heart rate, developmental rate, hatching success, and viability in medaka embryos. Within 4 days of exposure to doses > or = 40 pg AZA-1/egg, substantial retardation in development was observed as reduced somatic growth and yolk absorption, and delayed onset of blood circulation and pigmentation. Embryos treated to > or =40 pg AZA-1/egg had slower heart rates (bradycardia) for the 9 days in ovo period, followed by reduced hatching success. Microinjection of a contaminated mussel (Mytilus edulis) extract containing AZAs (AZA-1, -2, and -3), okadaic acid, and dinophysistoxin-2 resulted in similar responses from the fish embryos at equivalent doses. These studies demonstrate that AZA-1 is a potent teratogen to finfish. This work will complement future investigations on AZA-1 accumulation in marine food webs and provide a basis for understanding its toxicity at different trophic levels.
Jamie R Colman; Michael J Twiner; Philipp Hess; Terry McMahon; Masayuki Satake; Takeshi Yasumoto; Gregory J Doucette; John S Ramsdell
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2005-04-02
Journal Detail:
Title:  Toxicon : official journal of the International Society on Toxinology     Volume:  45     ISSN:  0041-0101     ISO Abbreviation:  Toxicon     Publication Date:  2005 Jun 
Date Detail:
Created Date:  2005-05-20     Completed Date:  2005-09-16     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  1307333     Medline TA:  Toxicon     Country:  England    
Other Details:
Languages:  eng     Pagination:  881-90     Citation Subset:  IM    
Marine Biotoxins Program, Center for Coastal Environmental Health and Biomolecular Research, NOAA/National Ocean Service, Charleston, SC 29412, USA.
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MeSH Terms
Bivalvia / chemistry
Embryo, Nonmammalian / drug effects*,  physiology
Embryonic Development / drug effects
Heart Rate / drug effects
Marine Toxins / isolation & purification,  toxicity*
Oryzias / embryology
Spiro Compounds / isolation & purification,  toxicity*
Teratogens / isolation & purification,  toxicity*
Reg. No./Substance:
0/Marine Toxins; 0/Spiro Compounds; 0/Teratogens; 0/azaspiracid

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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