Document Detail

Tensile stimuli increase nerve growth factor in human dermal fibroblasts independent of tension-induced TGFβ production.
MedLine Citation:
PMID:  23278900     Owner:  NLM     Status:  In-Data-Review    
Human dermal fibroblasts (HDFs) regulate wound-healing processes in human skin, including the regeneration of skin sensory fibres, in response to various mechanical stimuli. Because nerve growth factor (NGF) has an essential role in sensory regeneration, we evaluated the possible association of NGF with mechanical stimulus-dependent cellular responses in HDFs. A cyclic tensile stimulus increased both NGF and transforming growth factor (TGF) β2 production, yet with different gene transcription and signal desensitization profiles. Neutralizing TGFβ with antibodies did not affect the tension-induced NGF upregulation, with significant inhibition of endogenous TGFβ2 transcription. The treatment with LY294002, SP600125 or U0126 hindered the tension-induced TGFβ2 upregulation, although the increase in NGF was regulated only by SP600125 or U0126, indicating the involvement of three signalling kinase pathways in the upregulation of TGFβ2. However, the upregulation of NGF was shown to be independent of PI3K, demonstrating the independent regulation of tension-induced NGF and TGFβ production in HDFs.
Mina Kim; Dong Wook Shin; Hyunjun Shin; Minsoo Noh; Jennifer H Shin
Publication Detail:
Type:  Letter    
Journal Detail:
Title:  Experimental dermatology     Volume:  22     ISSN:  1600-0625     ISO Abbreviation:  Exp. Dermatol.     Publication Date:  2013 Jan 
Date Detail:
Created Date:  2013-01-02     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9301549     Medline TA:  Exp Dermatol     Country:  Denmark    
Other Details:
Languages:  eng     Pagination:  72-4     Citation Subset:  IM    
Copyright Information:
© 2013 John Wiley & Sons A/S.
Department of Mechanical Engineering, Korea Advanced Institute of Science and Technology, Daejeon, Korea.
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