| Teneurin carboxy (C)-terminal associated peptide-1 inhibits alkalosis-associated necrotic neuronal death by stimulating superoxide dismutase and catalase activity in immortalized mouse hypothalamic cells. | |
| | |
MedLine Citation:
|
PMID: 17900539 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
The teneurins and the teneurin C-terminal-associated peptides (TCAP) are implicated in the regulation of neuron growth and differentiation. However, current observations suggest that TCAP-1 may also have a neuroprotective action during times of pH-induced cellular stress in the brain such as during hypoxia-ischemia and brain alkalosis. To test this hypothesis, we cultured a TCAP-1-responsive mouse hypothalamic cell line, N38, using media buffered at pHs 6.8, 7.4, 8.0 and 8.4 subsequently treated with 100 nM TCAP-1. TCAP-1 significantly inhibited the decline in cell proliferation at pHs 8.0 and 8.4 as determined by direct cell viability assays and decreased the incidence of cells showing necrotic morphology. In addition, TCAP-1 decreased the number of cells undergoing necrosis by 4- to 5-fold as measured by uptake of ethidium homodimer III. Moreover, TCAP-1 significantly decreased the incidence of superoxide radicals and increased superoxide dismutase 1 (SOD1) expression. These results were accompanied by an increase in the SOD copper chaperone expression and increased catalase activity and expression. The results indicate that TCAP may play a neuroprotective role during periods of pH stress by upregulating oxygen radical scavenging systems. Thus, the TCAP-teneurin system may be part of a mechanism to protect neurons during trauma, such as hypoxia and ischemia. |
| | |
Authors:
|
Gina Trubiani; Arij Al Chawaf; Denise D Belsham; Dalia Barsyte-Lovejoy; David A Lovejoy |
Related Documents
:
|
7765589 - High density culture of mammalian cells with dynamic perfusion based on on-line oxygen ... 15696509 - Role of nutrient supply on cell growth in bioreactor design for tissue engineering of h... 199619 - H2o2 release from human granulocytes during phagocytosis. relationship to superoxide an... 19902029 - Cardiomyocyte h9c2 cells exhibit differential sensitivity to intracellular reactive oxy... 2507339 - Dopa and oxygen inhibit proliferation of retinal pigment epithelial cells, fibroblasts ... 20636139 - Antioxidants counteract nicotine and promote migration via racgtp in oral fibroblast ce... 2732359 - Neurogenesis and cell death in the isthmic nuclei of the frog limnodynastes dorsalis. 8482359 - Growth control in a human colon carcinoma cell line mediated by cell-associated transfo... 17307169 - A prostaglandin d2 system in the human testis. |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2007-08-22 |
Journal Detail:
|
Title: Brain research Volume: 1176 ISSN: 1872-6240 ISO Abbreviation: Brain Res. Publication Date: 2007 Oct |
Date Detail:
|
Created Date: 2007-10-18 Completed Date: 2009-01-16 Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 0045503 Medline TA: Brain Res Country: Netherlands |
Other Details:
|
Languages: eng Pagination: 27-36 Citation Subset: IM |
Affiliation:
|
Department of Cell and Systems Biology, University of Toronto, Toronto, Ontario, Canada. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Alkalosis
/
complications,
enzymology,
physiopathology Animals Catalase / metabolism* Cell Line, Transformed Cell Survival / drug effects, physiology Cytoprotection / drug effects Free Radical Scavengers / metabolism Hydrogen-Ion Concentration Hypothalamus / drug effects, enzymology*, physiopathology Mice Necrosis / drug therapy*, prevention & control Nerve Tissue Proteins / metabolism*, pharmacology Neurons / drug effects, enzymology* Neuroprotective Agents / metabolism, pharmacology Oxidative Stress / drug effects, physiology Superoxide Dismutase / metabolism* Superoxides / metabolism Up-Regulation / drug effects, physiology |
| Chemical | |
Reg. No./Substance:
|
0/Free Radical Scavengers; 0/Nerve Tissue Proteins; 0/Neuroprotective Agents; 0/TCAP-1 protein, mouse; 11062-77-4/Superoxides; EC 1.11.1.6/Catalase; EC 1.15.1.-/superoxide dismutase 1; EC 1.15.1.1/Superoxide Dismutase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: The role of NMDA and GABAA receptors in the inhibiting effect of 3 MPa nitrogen on striatal dopamine...
Next Document: Administration of oral methylphenidate during adolescence prevents suppressive development of dopami...