Document Detail


Tenascin expression patterns and cells of monocyte lineage: relationship in human gliomas.
MedLine Citation:
PMID:  10658911     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Stromal extracellular matrix (ECM) components are thought to play an important role in regulating invasion of human gliomas. Macrophages and microglial cells may heavily influence the integrity of the extracellular compartment of gliomas, and the affected ECM may play a key role in regulating migratory activity of both tumor cells and macrophages/microglia. The aim of this investigation was to study immunohistochemically the expression patterns of four ECM components: fibronectin, laminin, collagen IV, and tenascin (TN) in human gliomas, with special attention to TN. Our main goal was to study the possible correlation between TN expression and macrophagic/microglial infiltration in gliomas. Altogether, 90 gliomas were studied. Tumors included 46 glioblastomas, 19 anaplastic gliomas, 22 low grade gliomas, and 3 pilocytic astrocytomas. Vascular TN prevailed in perinecrotic areas of glioblastomas, whereas interstitial TN was more often expressed distant from necrosis and in the ECM of anaplastic and low grade gliomas. Double staining with CD68 and anti-TN antibodies showed that macrophagic/microglial density was significantly higher in TN-positive areas of most of the glioblastomas and anaplastic gliomas, whereas microglial percentage from total number of CD68-positive cells was in most of the cases significantly higher in TN-negative areas. In addition, we saw a morphologically spatial correlation between higher densities of macrophagic/microglial infiltration and TN expression in perinecrotic areas in glioblastomas. Attachment of macrophages to TN-positive basement membrane zones of newly formed stromal blood vessels was evident. On the basis of our results, we conclude that TN may play a crucial role in regulating trafficking of cells of monocyte lineage in human gliomas.
Authors:
A Kulla; A Liigant; A Piirsoo; G Rippin; T Asser
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc     Volume:  13     ISSN:  0893-3952     ISO Abbreviation:  Mod. Pathol.     Publication Date:  2000 Jan 
Date Detail:
Created Date:  2000-02-15     Completed Date:  2000-02-15     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8806605     Medline TA:  Mod Pathol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  56-67     Citation Subset:  IM    
Affiliation:
Department of Pathology, University of Tartu, Estonia.
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MeSH Terms
Descriptor/Qualifier:
Brain Neoplasms / metabolism*,  pathology
Cell Lineage
Collagen / metabolism
Fibronectins / metabolism
Glioma / metabolism*,  pathology
Humans
Immunoenzyme Techniques
Laminin / metabolism
Macrophages / metabolism,  pathology
Microglia / metabolism,  pathology
Monocytes / metabolism*,  pathology
Tenascin / metabolism*
Chemical
Reg. No./Substance:
0/Fibronectins; 0/Laminin; 0/Tenascin; 9007-34-5/Collagen

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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