| Ten antibodies, six colors, twelve parameters: A multiparameter flow cytometric approach to evaluate leptomeningeal disease in B-cell non-Hodgkin's lymphomas. | |
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MedLine Citation:
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PMID: 22328059 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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BACKGROUND: Flow cytometry (FC) is considered a sensitive and specific technique for the detection of occult lymphoma cells in cerebrospinal fluid (CSF). METHODS: The diagnostic sensitivity of a FC approach which uses a combination of 10 antibodies, a single-tube evaluation, and a six-color instrument, was evaluated and compared to conventional cytology (CC) for the detection of lymphomatous cells in the CSF of 44 patients affected by B-cell non-Hodgkin's lymphoma (B-NHL) considered at high risk of central nervous system spread. RESULTS: The CSF obtained from 36 newly diagnosed and 8 relapsed patients affected by B-cell lymphoma was assessed by FC and CC on a total of 62 samples; 52/62 (82.6%) were considered paucicellular as they had fewer than 10 cells/μl. All cases were evaluated by both methods. FC gave 15/62 (24%) positive results, CC 10/62 (16%) positive results; none of the samples evaluated had a positive CC with a negative FC result. CONCLUSIONS: The use of a multiparameter FC approach, which collects an elevated number of monoclonal antibodies in a single tube and identify different cell populations with a selective gating strategy analysis, allows for the evaluation of lymphocyte subsets and the detection of leptomeningeal disease in B-NHL, even in the presence of paucicellularity of samples. © 2012 International Clinical Cytometry Society. |
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Authors:
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Alessandra Stacchini; Sabrina Aliberti; Anna Demurtas; Giulia Benevolo; Laura Godio |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2011-11-14 |
Journal Detail:
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Title: Cytometry. Part B, Clinical cytometry Volume: - ISSN: 1552-4957 ISO Abbreviation: - Publication Date: 2011 Nov |
Date Detail:
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Created Date: 2012-2-13 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101235690 Medline TA: Cytometry B Clin Cytom Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
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Copyright © 2012 International Clinical Cytometry Society. |
Affiliation:
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Flow Cytometry Unit, Department of Pathology, Molinette Hospital, Turin Italy. astacchini@molinette.piemonte.it. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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