Document Detail

Temporospatial changes of kinin b2 receptors during the estrous cycle and pregnancy in the rat uterus.
MedLine Citation:
PMID:  11369583     Owner:  NLM     Status:  MEDLINE    
Tissue kallikreins are present in rat uterus during the estrous cycle in luminal and glandular epithelium, in early gestation in the implantation node, and in the last third of pregnancy surrounding the sinusoids in the decidua basalis. The pattern of kinin B2 receptor expression, through which the vasoactive effect of kallikreins is exerted, was studied by in vitro autoradiography and immunohistochemistry. The kinin B2 receptor was observed in the luminal and glandular epithelium, myometrium, endothelial cells of arteries, veins and venules, and smooth muscle cells of endometrial and myometrial arterioles. Immunoblotting of crude membranes revealed a band of 69 kDa that increased in late proestrus and estrus, concordantly with the pattern of immunostaining observed in the tissue. At Day 7 of gestation, the kinin B2 receptor was expressed (binding sites and receptor protein) in the epithelium of the implantation node and decidual cells; these latter cells showed a further increase during gestational Days 9 and 10. From Days 14 to 21, the subplacental decidua became strongly immunoreactive, and on Days 16 and 21 the placental labyrinthine endothelium was intensely stained. During this period, endothelium of arteries and veins, smooth muscular cells of small diameter arterioles, and myometrium also expressed B2 receptors. In unilaterally oil-stimulated pseudopregnancy, the decidual cells and the glandular epithelium show similar immunoreactivity to that during pregnancy. The temporospatial pattern of kinin B2 receptors, coinciding with that of kallikrein or with sites accessible to the generated kinins, further supports an autocrine-paracrine role for the kallikrein-kinin system in the vasoactive changes of implantation and placental blood flow regulation.
C D Figueroa; C Chacón; J Corthorn; P Ehrenfeld; W Müller-Esterl; G Valdés
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biology of reproduction     Volume:  64     ISSN:  0006-3363     ISO Abbreviation:  Biol. Reprod.     Publication Date:  2001 Jun 
Date Detail:
Created Date:  2001-05-22     Completed Date:  2001-08-02     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0207224     Medline TA:  Biol Reprod     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1590-9     Citation Subset:  IM    
Instituto de Histología y Patología, Universidad Austral, Valdivia, Chile.
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MeSH Terms
Bradykinin / metabolism
Cell Membrane / chemistry,  metabolism
Endothelium, Vascular / chemistry
Epithelium / chemistry
Estrus / metabolism*
Gestational Age
Muscle, Smooth, Vascular
Myometrium / chemistry
Pregnancy, Animal / metabolism*
Pseudopregnancy / metabolism
Rats, Sprague-Dawley
Receptor, Bradykinin B2
Receptors, Bradykinin / analysis,  metabolism*
Tissue Distribution
Uterus / chemistry,  metabolism*
Reg. No./Substance:
0/Receptor, Bradykinin B2; 0/Receptors, Bradykinin; 58-82-2/Bradykinin

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