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Temporary inhibition of Moloney-murine sarcoma virus (M-MSV) induced-tumours by adoptive transfer of ricin-treated T-lymphocytes.
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MedLine Citation:
PMID:  2437947     Owner:  NLM     Status:  MEDLINE    
The potential use of tumour-specific T-lymphocytes loaded with ricin in cell targeting experiments was investigated. Moloney-murine sarcoma virus (M-MSV)-specific T-lymphocytes, obtained in mass mixed leucocyte-tumour cell culture (MLTC) and a M-MSV-specific cytotoxic T-lymphocyte (CTL) clone, were incubated with 125I-labelled ricin in order to evaluate toxin uptake and release. The internalized ricin (4.5 X 10(-17) mol and 6.5 X 10(-17) mol per 10(2) MLTC and CTL clone cells, respectively) was released rapidly during the first 30 min following treatment, and at a constant but slower rate over the next few hours. The cytotoxic activity of ricin-treated cells evaluated against antigen-related target cells, in a short term incubation 51Cr release assay, was unaffected during the first 30 min after treatment but decreased with time over the next few hours. However, the growth of antigen related as well as of unrelated tumour cells was strongly inhibited by the addition of ricin-treated cells to the culture system, thus indicating that released ricin is toxic for untreated target cells. The in vivo localization pattern of ricin-treated radiolabelled MLTC cells was found to be comparable with that of untreated cells 1 h after i.v. injection into syngeneic sublethally irradiated mice. After 6 h, however, more radiolabel was recovered from the liver of mice receiving ricin-treated MLTC cells. Ricin-treated M-MSV-specific T-lymphocytes were injected i.v. into tumour bearing sublethally irradiated mice. A temporary tumour growth inhibition (up to 6 days) was achieved following transfer of low doses of ricin-treated MLTC or CTL clone cells (1 X 10(6) and 0.5 X 10(6), respectively). In contrast, in M-MSV injected control mice, receiving only free toxin (from 5 to 20 ng) or untreated tumour-specific effector cell tumours grew progressively. The therapeutic effect was apparently specific since the injection of ricin-treated alloreactive T-lymphocytes did not influence tumour growth. These results suggest that M-MSV-specific T-lymphocytes loaded with ricin can deliver toxin to the target tumour mass and have a transient therapeutic effect.
V Cerundolo; P Zanovello; D McIntosh; R Fabbris; A J Davies; D Collavo
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  British journal of cancer     Volume:  55     ISSN:  0007-0920     ISO Abbreviation:  Br. J. Cancer     Publication Date:  1987 Apr 
Date Detail:
Created Date:  1987-07-17     Completed Date:  1987-07-17     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  0370635     Medline TA:  Br J Cancer     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  413-9     Citation Subset:  IM    
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MeSH Terms
Cell Survival / drug effects
Epitopes / immunology
Immunization, Passive*
Lymphocyte Culture Test, Mixed
Mice, Inbred C57BL
Moloney murine sarcoma virus / immunology
Ricin / administration & dosage*,  therapeutic use
Sarcoma, Experimental / immunology,  prevention & control*
T-Lymphocytes, Cytotoxic / immunology*
Time Factors
Reg. No./Substance:
0/Epitopes; 9009-86-3/Ricin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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Journal Information
Journal ID (nlm-ta): Br J Cancer
ISSN: 0007-0920
ISSN: 1532-1827
Article Information
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Print publication date: Month: 4 Year: 1987
Volume: 55 Issue: 4
First Page: 413 Last Page: 419
ID: 2001701
PubMed Id: 2437947

Temporary inhibition of Moloney-murine sarcoma virus (M-MSV) induced-tumours by adoptive transfer of ricin-treated T-lymphocytes.
V. Cerundolo
P. Zanovello
D. McIntosh
R. Fabbris
A. J. Davies
D. Collavo

Article Categories:
  • Research Article

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