| Temporal variation in the expression of the p53 protein in proliferating and differentiating murine erythroleukaemia cells. | |
| | |
MedLine Citation:
|
PMID: 16896537 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Temporal changes in the expression of p53 were investigated during hexamethylene bisacetamide (HMBA)-induced differentiation of murine erythroleukaemic (MEL) cells. Cell preparations were analysed by SDS-PAGE and western immunoblotting, which detected an immunospecific band of molecular mass 53 kDa. This analysis provided semi-quantitative information. Cell extracts were analysed further by means of ELISA techniques, using a p53-specific antibody, to provide quantitative data. In time series experiments in which cells were isolated at 15, 30 and 60 min intervals, dynamic variations in the expression of the p53 protein were detected in both the untreated and the HMBA-treated MEL cells. In all cases, the effects were complex with variations in amplitude, frequency and phasing of the rhythms. The available evidence suggests that the observed patterns, like periodic variations in other systems, are modified in rhythmic fashion with respect to period and amplitude. The results add further support for our view that it is essential to consider the dynamics when interpreting the role of p53 and cellular processes in general. |
| | |
Authors:
|
U M Bodalina; K D Hammond; D A Gilbert |
Related Documents
:
|
8453637 - P53 mutations selected in vivo when mouse mammary epithelial cells form hyperplastic ou... 18434687 - Association of high proliferation in adult t-cell leukemia cells with apoptosis, and ex... 7915517 - Transcriptional regulation of the pcna promoter by p53. 9698077 - 5-fluorouracil-mediated thymidylate synthase induction in malignant and nonmalignant hu... 3700797 - Somatic cell counts and infection rates for cows of varying somatic cell count in initi... 490057 - Distribution of 3-hydroxy-3-methylglutaryl coenzyme a reductase and alkaline phosphatas... |
Publication Detail:
|
Type: Journal Article Date: 2006-07-29 |
Journal Detail:
|
Title: Molecular and cellular biochemistry Volume: 294 ISSN: 0300-8177 ISO Abbreviation: Mol. Cell. Biochem. Publication Date: 2007 Jan |
Date Detail:
|
Created Date: 2007-01-22 Completed Date: 2007-04-11 Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 0364456 Medline TA: Mol Cell Biochem Country: Netherlands |
Other Details:
|
Languages: eng Pagination: 155-62 Citation Subset: IM |
Affiliation:
|
Department of Molecular Medicine and Haematology, Medical School, University of the Witwatersrand, York Road, Parktown, Johannesburg, 2193, South Africa. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Acetamides
/
pharmacology Animals Antineoplastic Agents / pharmacology Cell Differentiation* Cell Line, Tumor Cell Proliferation* Enzyme-Linked Immunosorbent Assay Leukemia, Erythroblastic, Acute / physiopathology* Mice Time Factors Tumor Suppressor Protein p53 / genetics, metabolism* |
| Chemical | |
Reg. No./Substance:
|
0/Acetamides; 0/Antineoplastic Agents; 0/Tumor Suppressor Protein p53; 3073-59-4/hexamethylene bisacetamide |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: The NADPH- and iron-dependent lipid peroxidation in human placental microsomes.
Next Document: Therapeutic efficacy of Kalpaamruthaa on reactive oxygen/nitrogen species levels and antioxidative s...