| Temporal variation of hydroxyl radical generation and 8-hydroxy-2'-deoxyguanosine formation by coarse and fine particulate matter. | |
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MedLine Citation:
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PMID: 12709515 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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AIMS: To determine the induction of 8-hydroxy-2'-deoxyguanosine (8-OHdG) by fine (<2.5 microm) and coarse (10-2.5 microm) particulate matter (PM) sampled over time at one sampling location, and to relate the observed effects to the hydroxyl radical (*OH) generating activities and transition metal content of these samples, and to meteorological parameters. METHODS: Weekly samples of coarse and fine PM were analysed for H(2)O(2) dependent *OH formation using electron spin resonance (ESR) and formation of 8-OHdG in calf thymus DNA using an immuno-dotblot assay. Immunocytochemistry was used to determine 8-OHdG formation in A549 human epithelial lung cells. To determine temporal effects, samples from six weeks in summer and six weeks in autumn/winter were compared using ESR and the dotblot assay. Concentrations of leachable V, Cr, Fe, Ni, and Cu were determined by inductively coupled plasma mass spectrometry. RESULTS: Both PM fractions elicited *OH generation as well as 8-OHdG formation in calf thymus DNA and in A549 cells. 8-OHdG formation in the naked DNA was significantly related to *OH generation, but not to metal concentrations except for copper. A significantly higher *OH generation was observed for coarse PM, but not fine PM collected during the autumn/winter season; this was not due to differences in sampled mass or metal content. Specific weather conditions under which increased *OH formation in the coarse mode was observed suggest that other, as yet unknown, anthropogenic components might affect the radical generating capacity of PM. CONCLUSIONS: Both coarse and fine PM are able to generate *OH, and induce formation of 8-OHdG. When considered at equal mass, *OH formation shows considerable variability with regard to the fraction of PM, as well as the sampling season. The toxicological implications of this heterogeneity in *OH formation by PM, as can be easily determined by ESR, need further investigation. |
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Authors:
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T Shi; A M Knaapen; J Begerow; W Birmili; P J A Borm; R P F Schins |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Occupational and environmental medicine Volume: 60 ISSN: 1351-0711 ISO Abbreviation: Occup Environ Med Publication Date: 2003 May |
Date Detail:
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Created Date: 2003-04-23 Completed Date: 2003-05-22 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 9422759 Medline TA: Occup Environ Med Country: England |
Other Details:
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Languages: eng Pagination: 315-21 Citation Subset: IM |
Affiliation:
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Institut für umweltmedizinische Forschung (IUF) an der Heinrich-Heine-Universität Düsseldorf gGmbH, Auf'm Hennekamp 50, D-40225 Düsseldorf, Germany. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cattle DNA / metabolism Deoxyadenosines / metabolism* Dose-Response Relationship, Immunologic Electron Spin Resonance Spectroscopy Hydroxyl Radical / chemistry*, metabolism Meteorological Concepts Particle Size Thymus Gland / metabolism |
| Chemical | |
Reg. No./Substance:
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0/8-hydroxy-2'-deoxyadenosine; 0/Deoxyadenosines; 3352-57-6/Hydroxyl Radical; 9007-49-2/DNA |
| Comments/Corrections | |
Comment In:
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Occup Environ Med. 2003 May;60(5):313-4
[PMID:
12709514
]
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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