Document Detail


Temporal and spatial patterns of glial differentiation in the surgically induced spinal open neural tube defect of chick embryos: astrocytic, radial glial and microglial differentiations.
MedLine Citation:
PMID:  12483353     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
INTRODUCTION: There are controversies over the pattern of glial differentiation in spinal open neural tube defect (ONTD) at the prenatal stage. A surgical model of ONTD allows a more precise comparison of glial differentiation between the ONTD and control groups than chemical and genetic models. MATERIALS AND METHODS: To investigate the influence of ONTDs on the patterns of glial differentiation, ONTDs were induced by surgery using Hamburger and Hamilton stage 18 or 19 chick embryos. The spinal cord tissues on postoperative days (POD) 5, 7, 10, and 14 were processed to observe astrocytic, radial glial, and microglial differentiations by glial fibrillary acid protein (GFAP), vimentin and ricinus communis agglutinin-I (RCA-I) stainings, respectively. Four embryos were assigned to subgroups of each POD. Control embryos ( n=4) were staged but the neural tubes were not incised. RESULTS: In the control group, GFAP positivity was shown faintly at the dorsal midline on embryonic day (E) 10 (corresponding to POD 7), in the ventral one-third of the white matter on E 13 and in the whole white matter on E 17. Embryos with ONTDs showed earlier and stronger GFAP positivity from POD 7-14, especially at the dorsal surface and the adjacent gray matter. In the control group, vimentin staining demonstrated a positive reaction at the midline with positivity in a faint, radial pattern on E 8 and E 10. This had all disappeared by E 13 and 17. In embryos with ONTDs, vimentin positivity was enhanced and persisted from POD 5-14. These findings were prominent along the dorsal surface of ONTDs. No difference in RCA-I staining was found between the control and ONTD groups. CONCLUSION: The results reveal that ONTD promotes astrocytic differentiation and prolongs expression of radial glial fibers, which seems to be a reaction to the damage caused by exposure of the spinal cord tissue to amniotic fluid.
Authors:
Ki-Bum Sim; You-Nam Chung; Sa-Sun Cho; Byung-Kyu Cho; Martha Kim; Dong Won Kim; Yong Do Huh; Kyu-Chang Wang
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't     Date:  2002-10-09
Journal Detail:
Title:  Child's nervous system : ChNS : official journal of the International Society for Pediatric Neurosurgery     Volume:  18     ISSN:  0256-7040     ISO Abbreviation:  Childs Nerv Syst     Publication Date:  2002 Dec 
Date Detail:
Created Date:  2002-12-16     Completed Date:  2003-04-08     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8503227     Medline TA:  Childs Nerv Syst     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  694-701     Citation Subset:  IM    
Affiliation:
Department of Neurosurgery, Cheju National University College of Medicine, Cheju, Korea.
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MeSH Terms
Descriptor/Qualifier:
Animals
Astrocytes / metabolism,  pathology
Cell Differentiation
Chick Embryo
Disease Models, Animal
Glial Fibrillary Acidic Protein / metabolism
Immunohistochemistry
Neural Tube Defects / embryology,  pathology*
Neuroglia / metabolism,  pathology*
Plant Lectins
Spinal Cord / abnormalities*,  embryology,  surgery
Staining and Labeling
Time Factors
Vimentin
Chemical
Reg. No./Substance:
0/Glial Fibrillary Acidic Protein; 0/Plant Lectins; 0/Ricinus communis agglutinin-1; 0/Vimentin

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