Document Detail


Temporal and spatial expression of c-jun and jun-B proto-oncogenes in pulp cells involved with reparative dentinogenesis after cavity preparation of rat molars.
MedLine Citation:
PMID:  10029466     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
c-jun and jun-B are nuclear proto-oncogenes induced by growth factors such as bone morphogenetic proteins (BMPs). These gene products enhance the expression of many genes, including osteocalcin and collagen types, indicating that c-jun and jun-B play important roles in the cell differentiation process. It is also known that BMPs affect the differentiation of pulp cells to odontoblast-like cells during reparative dentinogenesis, but little is known about the transcriptional regulation of genes in cells associated with reparative dentinogenesis. In this study, we examined the expression of c-jun and jun-B in pulp cells during reparative dentinogenesis after cavity preparation of rat molars by in situ hybridization. In rat tooth germs, c-jun and jun-B were co-expressed in the odontoblastic lineage. In rat adult molars, c-jun was expressed in the odontoblast layer, but the jun-B expression was absent in all pulp cells. After cavity preparation, we found that c-jun and jun-B were coexpressed in pulp cells underneath cavities. During the early phase of reparative dentinogenesis, levels of c-jun and jun-B greatly increased in pulp cells within and around the reparative dentin matrix formed adjacent to the cavity floor. Fourteen days after cavity preparation, c-jun and jun-B were expressed only in pulp cells lining the irregular surface of the thick reparative dentin. These results suggest that c-jun and jun-B may play important roles both in physiological and in reparative dentinogenesis; in particular, the limited distribution of the jun-B expression suggests a specific role of jun-B only in cells involved with the active formation of the dentin matrix during primary and reparative dentinogenesis.
Authors:
C Kitamura; K Kimura; T Nakayama; M Terashita
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of dental research     Volume:  78     ISSN:  0022-0345     ISO Abbreviation:  J. Dent. Res.     Publication Date:  1999 Feb 
Date Detail:
Created Date:  1999-02-23     Completed Date:  1999-02-23     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0354343     Medline TA:  J Dent Res     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  673-80     Citation Subset:  D; IM    
Affiliation:
Department of Operative Dentistry, Kyushu Dental College, Kitakyushu, Japan.
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MeSH Terms
Descriptor/Qualifier:
Animals
Dental Cavity Preparation
Dental Pulp / cytology
Dentin, Secondary / growth & development*
Dentinogenesis / genetics*
Gene Expression
Genes, jun / physiology*
In Situ Hybridization
Odontoblasts / physiology
RNA Probes
Rats
Rats, Wistar
Specific Pathogen-Free Organisms
Tooth Germ / physiology
Chemical
Reg. No./Substance:
0/RNA Probes

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